等级
certified by the Biological Stain Commission
质量水平
形式
powder
组成
dye content, ≥90%
mp
290 °C (dec.) (lit.)
溶解性
H2O: 1 mg/mL
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChI key
RZSYLLSAWYUBPE-UHFFFAOYSA-L
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应用
固绿 FCF已用于：
- 与番红花素一起用于结节切片染色
- 胶原沉积染色程序中
- 蛋白质和淀粉的染色
- 作为染料检测和注射鸡胚 DNA 溶液
固绿 FCF 染料用于 IEF 中的蛋白质染色，天然 PAGE 和 SDS-PAGE。 固绿染色在比亮蓝 R 更广泛的蛋白质浓度范围内呈线性。为了使灵敏度最高，建议电泳后将蛋白质固定在凝胶中。 然后用 0.1％ 固绿 FCF 在 30％（v/v）乙醇，10％（v/v）乙酸或 7％ 乙酸中将凝胶染色 2 小时。 凝胶可以用 30％（v/v）乙醇，10％（v/v）乙酸或 7％ 乙酸脱色。 可以在 625nm 处扫描染色的凝胶。 检测灵敏度约为亮蓝 R 的 30％。
适用性
番红固绿 FCF 植物细胞染色剂认证；作为 Gomori一步三色程序的苯胺蓝替代品。
警示用语：
Warning
危险声明
危险分类
Muta. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 2
个人防护装备
Eyeshields, Gloves
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