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About This Item
经验公式（希尔记法）:
C44H47N3O24
CAS号:
分子量:
1001.85
Beilstein:
8183748
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47
推荐产品
产品名称
Fura 2-AM, ≥95% (HPLC)
质量水平
方案
≥95% (HPLC)
表单
powder
颜色
yellow to yellow-green
溶解性
acetone: 10 mg/mL
应用
diagnostic assay manufacturing
hematology
histology
储存温度
−20°C
SMILES字符串
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c4ncc(o4)C(=O)OCOC(C)=O
InChI
1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3
InChI key
VPSRLGDRGCKUTK-UHFFFAOYSA-N
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一般描述
Fura 2- am（Fura 2）是一种钙特定比率荧光染料。它也被称为乙酰氧基甲酯。 为了响应钙离子，像Fura 2这样的比例度量染料改变了各自的激发或发射光谱。细胞内钙浓度由不同波长的荧光发射比或激发决定。Fura 2的发射峰在505nm，激发峰在340-380nm之间，是首选的钙指示剂。
应用
Fura 2-AM是一种常用的探针，用于测定完整活细胞中细胞质游离钙的动态变化。
特点和优势
使用Fura 2的酯形式作为Fura 2-AM的主要好处有：可渗透细胞。容易载入细胞中。与单波长染料相比，使用Fura 2-AM的另一个优点是，比率信号与以下因素无关：染料浓度。照明强度、光程长度。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
J L Donato et al.
British journal of pharmacology, 119(7), 1447-1453 (1996-12-01)
1. Proteoglycans provide negatively charged sites on the surface of platelets, leukocytes and endothelial cells. Since chondroitin 4-sulphate is the main proteoglycan present on the platelet surface, the role of this molecule in mediating the activation of human platelets by
Effects of Taurine or Trehalose supplementation on functional competence of cryopreserved Karan Fries semen.
Chhillar S
Animal Reproduction, 135, 1-1 (2012)
Assessment of intracellular Ca2+, cAMP and 1,2-diacylglycerol in cryopreserved buffalo (Bubalus bubalis) spermatozoa on supplementation of taurine and trehalose in the extender.
Singh VK
Reproduction in domestic animals = Zuchthygiene, 47, 584-584 (2012)
M W Roe et al.
Cell calcium, 11(2-3), 63-73 (1990-02-01)
Fura-2 has become the most popular fluorescent probe with which to monitor dynamic changes in cytosolic free calcium in intact living cells. In this paper, we describe many of the currently recognized limitations to the use of Fura-2 in living
B T Kinsella et al.
The Journal of pharmacology and experimental therapeutics, 281(2), 957-964 (1997-05-01)
To establish whether the thromboxane A2 (TXA2) receptor (TP) functionally couples to the Gq family of heterotrimeric G proteins in vivo, we have coexpressed the cDNAs coding for the human platelet/placental TP alpha isoform (TP alpha) and the alpha subunits
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