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D9564

Sigma-Aldrich

DAPI，二乳酸

≥98% purity (HPLC), powder

别名:

4 & # 8242;,6-二脒基-2-苯吲哚,二乳酸酯

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About This Item

经验公式（希尔记法）:
C16H15N5 · 2C3H6O3
分子量:
457.48
UNSPSC代码:
12171500
PubChem化学物质编号:
329798905
NACRES:
NA.47

product name

DAPI，二乳酸, ≥98% (HPLC)

质量水平

300

检测方案

≥98% (HPLC)

形式

powder

颜色

yellow

溶解性

H2O: 20 mg/mL

ε (消光系数)

28.00-32.50 at 345-351 nm in methanol

应用

diagnostic assay manufacturing
hematology
histology

储存温度

2-8°C

SMILES字符串

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)

InChI key

LCAUPANIXBMNIA-UHFFFAOYSA-N

相关类别

一般描述

DAPI是一种吲哚林染料。它携带两种脒成分，一种在苯基环上，另一个直接在吲哚部分上。

应用

DAPI二乳酸盐已用于细胞的核染色。DAPI是一种常用的流式细胞术染色剂，广泛应用于DNA和核染色。此外，它还用于原位杂交和其他免疫荧光程序后的复染剂。

生化/生理作用

一种用于检测单个核酸分子的高灵敏度染料。DAPI已经被证明能检测溶液中的单一的脱氧核糖核酸分子，并能检测单细胞和叶绿体中的脱氧核糖核酸图谱。

相关产品

产品编号
说明
价格

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

分析证书(COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. Which filter sets can be used to visualize the fluorophore produced by Product D9564, DAPI, dilactate (DAPI-DNA)?

    One such filter set is the Zeiss Filter Set 01 (ex=359 nm, em=461 nm), information about which is posted here.

  5. Isn't the hydrochloride salt form of DAPI a more sensitive detector of DNA than Product D9564, DAPI, dilactate (DAPI-DNA)?

    The dilactate salt has as much sensitivity in detection as the dihydrochloride salt, which we also offer as Product No. D9542.

  6. Can Product D9564, DAPI dilactate (DAPI-DNA), be used as a vital stain?

    As we note in the Product Information Sheet for the Dihydrochloride salt of DAPI, Product No. D9542, DAPI can be used as a vital dye to stain mature pollen grains, but we have tested it as a vital stain in other cell types.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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