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Merck
CN

B9431

生物素-4-荧光素

≥95% purity (HPLC), powder

别名:

5-(6)-[2-(生物素)氨基乙基氨基羰基]荧光素, B4F, 生物素（5-荧光素）偶合物

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关于此项目

经验公式（希尔记法）:
C33H32N4O8S
分子量:
644.69
MDL number:
MFCD09264223
UNSPSC Code:
12171500
eCl@ss:
34058011
PubChem Substance ID:
329773319
NACRES:
NA.47

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产品名称

生物素-4-荧光素, ≥95.0% (HPLC)

InChI key

UROXVNHIYQXTRA-LFERIPGTSA-N

InChI

1S/C33H32N4O8S/c38-18-7-10-25-23(14-18)29(21-9-6-19(39)15-26(21)45-25)20-8-5-17(13-22(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30

SMILES string

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56

assay

≥95.0% (HPLC)

form

powder

mol wt

644.71

technique(s)

titration: suitable

color

light orange to very dark orange
light yellow to very dark yellow

ε (extinction coefficient)

≥70 at 491-497 nm in phosphate buffer at 0.05 M

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100
200

相关类别

Application

可用于定量生物素结合位点。该试剂的亲和素结合速度比常规荧光素生物素更快。
生物素-4-荧光素已被用于标记亲和素，以研究碳阴离子植入亲和素后蛋白质结合能力的变化。它已被用于研究链霉亲和素对生物素的结合亲和力。

Biochem/physiol Actions

生物素 - 荧光素主要用于检测亲和素和链霉亲和素中的生物素相互作用位点。生物素-4-荧光素的结合将导致强淬灭，这可用于测量（链霉）亲和素的浓度。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCV3163
MKCT4294
MKCS3735
MKCP6327
MKCP0536

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