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Merck
CN

B9431

Sigma-Aldrich

生物素-4-荧光素

≥95% purity (HPLC), powder

别名:

5-(6)-[2-(生物素)氨基乙基氨基羰基]荧光素, B4F, 生物素(5-荧光素)偶合物

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About This Item

经验公式(希尔记法):
C33H32N4O8S
分子量:
644.69
MDL编号:
UNSPSC代码:
12171500
eCl@ss:
34058011
PubChem化学物质编号:
NACRES:
NA.47

产品名称

生物素-4-荧光素, ≥95.0% (HPLC)

质量水平

100
200

方案

≥95.0% (HPLC)

表单

powder

分子量

644.71

技术

titration: suitable

颜色

light orange to very dark orange
light yellow to very dark yellow

ε (消光系数)

≥70 at 491-497 nm in phosphate buffer at 0.05 M

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56

InChI

1S/C33H32N4O8S/c38-18-7-10-25-23(14-18)29(21-9-6-19(39)15-26(21)45-25)20-8-5-17(13-22(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30

InChI key

UROXVNHIYQXTRA-LFERIPGTSA-N

应用

生物素-4-荧光素已被用于标记亲和素,以研究碳阴离子植入亲和素后蛋白质结合能力的变化。它已被用于研究链霉亲和素对生物素的结合亲和力。
可用于定量生物素结合位点。该试剂的亲和素结合速度比常规荧光素生物素更快。

生化/生理作用

生物素 - 荧光素主要用于检测亲和素和链霉亲和素中的生物素相互作用位点。生物素-4-荧光素的结合将导致强淬灭,这可用于测量(链霉)亲和素的浓度。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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分析证书(COA)

Lot/Batch Number

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访问文档库

Sivaraj Sivaramakrishnan et al.
The Journal of cell biology, 187(1), 53-60 (2009-09-30)
Unconventional myosins interact with the dense cortical actin network during processes such as membrane trafficking, cell migration, and mechanotransduction. Our understanding of unconventional myosin function is derived largely from assays that examine the interaction of a single myosin with a
Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization.
Kada G, et al.
Biochimica et Biophysica Acta, 1427, 44-48 (1999)
Cell-Adhesion Patterning by using Carbon Negative-Ion Implantation into Albumin and Avidin Layers on Polystyrene.
Sommani P, et al.
Transactions of the Materials Research Society of Japan, 36.3, 289-292 (2011)
Nathaniel G Hentz et al.
Journal of laboratory automation, 19(2), 153-162 (2013-09-14)
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays

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