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B4380

Sigma-Aldrich

溴乙烷

≥97% purity, powder

别名:

单溴代二苯甲烷

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About This Item

经验公式（希尔记法）:
C10H11BrN2O2
CAS号:
71418-44-5
分子量:
271.11
Beilstein:
4430959
MDL编号:
MFCD00036951
UNSPSC代码:
12171500
PubChem化学物质编号:
24891769
NACRES:
NA.47

产品名称

溴乙烷, ≥97% (HPLC)

质量水平

200

方案

≥97% (HPLC)

表单

powder

颜色

yellow

mp

161 °C

溶解性

acetonitrile: 20 mg/mL

ε (消光系数)

4.6-5.1 at 396-398 nm in H2O

应用

diagnostic assay manufacturing
hematology
histology

储存温度

−20°C

SMILES字符串

CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O

InChI

1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3

InChI key

AHEWZZJEDQVLOP-UHFFFAOYSA-N

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相关类别

一般描述

溴代双满又称单溴联苯胺。它还是一种已知的硫醇探针，是一种在光解反应后活化的荧光试剂。

应用

溴代双满用于高效液相色谱法硫醇测定,适于荧光测定2，3-二巯基丙烷-1-磺酸和其他二硫醇。溴代双满已用作研究牛心线粒体寡霉素敏感性 ATP 酶的荧光标记。
此物质可用于体内和体外游离硫化氢的定量测定。它已用于标记含有硫醇基的蛋白质。

生化/生理作用

溶液形式的溴代双满可与小分子硫醇基（如 GSH）和反应性蛋白硫醇基（如血红蛋白）反应。溴代双满与硫醇的反应为二级pH依赖性反应，且在与硫醇盐反应后，可活化水溶性荧光产物以便检测。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000420688
0000420688
0000385726
0000369257
0000369257

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N S Kosower et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(7), 3382-3386 (1979-07-01)
The bimane fluorescent labels, monobromobimane, dibromobimane, and monobromotrimethylammoniobimane, are derivatives of syn-9,10-dioxabimane:1,5-diazabicyclo[3.3.0]octa-3,6-diene-2,8-dione. They efficiently label hemoglobin (reactive thiol groups), membrane proteins, and glutathione of normal human red cells under physiological conditions. Monobromobimane and dibromobimane are effective on intact cells while
Differing effects of mechanical dough development and sheeting development methods on aggregated glutenin proteins.
Sutton KH, et al.
Cereal Chem., 80.6, 707-711 (2003)
Joachim Volk et al.
Dental materials : official publication of the Academy of Dental Materials, 25(12), 1556-1563 (2009-09-01)
Camphorquinone (CQ) is cytotoxic in cell cultures. The mechanism of this toxic action, however, is not yet clearly understood. Aim of this investigation was to analyze the effects of non-irradiated CQ on intracellular formation of reactive oxygen species (ROS), intracellular
Florencia Sardi et al.
Analytical biochemistry, 435(1), 74-82 (2013-01-09)
A method based on the differential reactivity of thiol and thiolate with monobromobimane (mBBr) has been developed to measure nucleophilicity and acidity of protein and low-molecular-weight thiols. Nucleophilicity of the thiolate is measured as the pH-independent second-order rate constant of
Bromobimane probes for thiols.
E M Kosower et al.
Methods in enzymology, 251, 133-148 (1995-01-01)
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