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Merck
CN

861006

Sigma-Aldrich

伊红 B

certified by the Biological Stain Commission, Dye content 90 %

别名:

4′,5′-二溴-2′,7′-二硝基荧光素 二钠盐, Saffrosine, 伊红猩红, 伊红蓝, 酸性红91

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About This Item

线性分子式:
C20H6N2O9Br2Na2
CAS号:
分子量:
624.06
颜色索引号:
45400
Beilstein:
4121964
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

等级

certified by the Biological Stain Commission

质量水平

形式

powder

组成

Dye content, 90%

溶解性

H2O: 1 mg/mL, clear, red

λmax

395 nm
514 nm

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

InChI key

GYYTYUGGVBYJHE-UHFFFAOYSA-L

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应用

  • 曙红B已用于从大鼠获得的肛门括约肌切片的组织学分析。
  • 它已与Mayer′s苏木精一起用于胸骨三角肌切片的染色。
  • 它已与苏木精一起用于从小鼠获得的不同组织切片的染色。

生化/生理作用

曙红B是一种呫吨染料,也称为曙红蓝或赤藓红B。它用于Negri小体的Harris染色。曙红B还用于Mayer′s Hemalum中,作为胶原蛋白的复染剂。曙红B-天青A一起用于细胞颗粒、细胞核和微生物的染色。也可用于蛋白估计值。蛋白-曙红B复合物在536-544nm波长范围内吸收,蓝移的变化有助于确定蛋白的浓度。

适用性

在Mayer′s Hemalum之后对胶原蛋白进行复染。与天青A一起用作细胞颗粒,细胞核和微生物的组织染色剂。用于垂体前叶细胞的差异染色。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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