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Merck
CN

77619

Supelco

苯酚-氯仿-异戊醇混合物

BioUltra, for molecular biology, 125:24:1

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About This Item

MDL编号:
UNSPSC代码:
12000000
PubChem化学物质编号:
NACRES:
ND.02

等级

for molecular biology

质量水平

产品线

BioUltra

形式

liquid

杂质

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH值(酸碱度)

4-5(H2O-phase, after extraction with H2O, 1:1)

密度

1.12 g/mL at 20 °C

λ

neat

紫外吸收

λ: 330 nm Amax: 0.2
λ: 405 nm Amax: 0.1
λ: 510 nm Amax: 0.1

储存温度

2-8°C

SMILES字符串

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

InChI key

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

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应用


  • Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems: This research explores the application of the Phenol – chloroform – isoamyl alcohol mixture in extracting high-quality RNA for advanced therapeutic formulations, emphasizing its critical role in nanotechnology and pharmaceutical sciences (Lokras et al., 2022).

  • Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry: Demonstrates the use of the Phenol – chloroform – isoamyl alcohol mixture for precise extraction of microRNAs from biological fluids, contributing to cancer diagnostics and molecular pathology (Pero-Gascon et al., 2018).

  • The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs: Highlights the integration of the Phenol – chloroform – isoamyl alcohol mixture with novel technologies for forensic applications, enhancing DNA extraction processes critical in legal and criminal investigations (Nori and McCord, 2015).

  • Frequency of Epstein-Barr virus DNA sequences in human gliomas: Utilizes the Phenol – chloroform – isoamyl alcohol mixture for DNA extraction in medical research, facilitating the study of viral presence in cancer tissues and its implications for oncology and virology (Fonseca et al., 2015).

  • The critical role of DNA extraction for detection of mycobacteria in tissues: This study underscores the effectiveness of the Phenol – chloroform – isoamyl alcohol mixture in the molecular detection of pathogens, serving as a fundamental technique in microbiological diagnostics and infectious disease research (Radomski et al., 2013).

外形

苯酚:氯仿:异戊醇混合物,比例为 125:24:1(v/v/v);用2 M NaOAc(pH 4)饱和

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

靶器官

Liver,Kidney, Nervous system,Kidney,Liver,Skin

WGK

WGK 3

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

危险化学品
易制毒化学品(2类)

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分析证书(COA)

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Dae-Wook Yang et al.
Cell death and differentiation, 28(1), 233-250 (2020-08-02)
The microtubule network is crucial for cell structure and function. Patronin is a conserved protein involved in protecting the minus end of microtubules. Conversely, Klp10A is a kinesin-like microtubule depolymerase. Here we report the role of Drosophila Patronin and Klp10A
Wiesław Bogdanowicz et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(30), 12279-12282 (2009-07-09)
We report the results of mitochondrial and nuclear DNA analyses of skeletal remains exhumed in 2005 at Frombork Cathedral in Poland, that are thought to be those of Nicolaus Copernicus (1473-1543). The analyzed bone remains were found close to the

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