Assurance^® GDS MPX ID for Top STEC Kit

Name: Assurance<SUP>&#174;</SUP> GDS MPX ID for Top STEC Kit
Brand: SIAL

suitable for PCR testing in Food and Beverages applications

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About This Item

UNSPSC代码:

41106302

NACRES:

NA.85

product name

GDS MPX ID for Top STEC, BioControl, Molecular based secondary screening PCR test for the detection and identification of "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food and environmental samples

Agency

AOAC^® (PTM)

质量水平

200

保质期

6‑12 mo.

包装

pkg of 52 tests

制造商/商品名称

Assurance GDS^®
BioControl Systems 71019-52

技术

PCR: suitable

应用

food and beverages
pathogen testing

相容性

for use with GDS Rotor-Gene

储存温度

2-8°C

适用性

enterovirulent E. coli (EEC) (shiga toxigenic)

一般描述

Assurance^® GDS is an advanced genetic detection system for food pathogens. The MPX ID for Top STEC is a secondary automated nucleic acid amplification system for the identification of “Top Six” non-O157 Shiga Toxigenic E. coli (STEC). The Top 6 non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26, or O45 that possess both the eae gene and at least one of the Shiga Toxin genes (stx1 or stx2). GDS MPX ID utilizes a proprietary PickPen^® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 6 specific Top STEC O-serogroups prior to genetic analysis for the associated pathogenicity genes. Each of the 3 unique eae gene sequences targeted by MPX ID are highly correlated plus one or more of the 6 O-serogroups comprising Top 6 non-O157 STEC. Because there are 3 different eae gene targets and 6 different O-serogroup targets, the PCR reactions have been divided between 2 Amplification Tubes. Each tube contains an internal control and all necessary PCR reagents. Results will automatically be combined between the 2 tubes via GDS Rotor-Gene^® software.

应用

Assurance^® GDS MPX ID for Top STEC is designed to follow GDS MPX Top 7 STEC (PN 71015BC) for the identification of “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in a variety of foods, ingredients, and environmental samples. Additionally, GDS MPX ID can be used to confirm isolated colonies from samples presumptive positive for Top 6 STEC.

特点和优势

Designed specifically to follow GDS MPX Top STEC assays
Identification of the specific O serogroup(s) responsible for the positive STEC result and verifies that all targets relevant to the definition of a Top 6 STEC (eae, stx and O group) are present within a single organism
Confirm isolated colonies from samples presumptive positive for Top 6 STEC
Utilizes the same single-step mEHEC^® enrichment media common to all BioControl methods for Salmonella, E.coli O157:H7, Shiga Toxin genes, and Top STEC
Only 14 hours from start to finish