一般描述
GDS MPX Top 6 STEC is an automated nucleic acid amplification system for the detection of the “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in raw ground beef, raw beef trim, raw spinach, and raw mixed greens (baby lettuce and other young leafy green vegetables). The "Top Six" non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26 or O45 that possess both the eae gene and at least one of the Shiga Toxin Genes stx1 or stx2. GDS MPX Top 6 STEC utilizes a proprietary IMS-based sample preparation procedure to isolate organisms belonging to 6 specific Top STEC O-serogroups (O103, O111, O121, O145, O26 and O45) prior to genetic analysis for the associated pathogenicity genes.
特点和优势
- Following sample preparation with IMS against the Top 6 O groups, samples are analyzed with a single multiplex assay for the presence of (eae), stx1, and stx2
- Single-step 10-18 h enrichment in mEHEC®, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC
组分
- MPX Top STEC Amplification Tubes
- Top 6 STEC Concentration Reagent
- Resuspension Buffer Tq
- Top STEC Wash Solution
相容性
BioControl GDS
测试方法
PCR, Immunomagnetic Separation (IMS)
法律信息
mEHEC is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
预防措施声明
危险分类
Met. Corr. 1
储存分类代码
8A - Combustible corrosive hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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