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Merck
CN
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文件

69899

Sigma-Aldrich

MBCL [单氯二胺]

suitable for fluorescence, ≥70.0% (HPCE)

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别名:
mBCl, MBCL [单氯二胺]
经验公式（希尔记法）:
C10H11ClN2O2
CAS号:
76421-73-3
分子量:
226.66
Beilstein:
4440901
MDL编号:
MFCD00077379
UNSPSC代码:
12352108
PubChem化学物质编号:
329762099
NACRES:
NA.32

质量水平

100

检测方案

≥70.0% (HPCE)

形式

powder

mp

135-136 °C (lit.)

溶解性

DMF: soluble
DMSO: soluble
acetonitrile: soluble
methanol: soluble

荧光

λex 380 nm; λem 461 nm in methanol
λex 390 nm; λem 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione)

适用性

suitable for fluorescence

SMILES字符串

CC1=C(C)C(=O)N2N1C(CCl)=C(C)C2=O

InChI

1S/C10H11ClN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3

InChI key

SUIPVTCEECPFIB-UHFFFAOYSA-N

一般描述

单氯二胺是一种可渗透入细胞的谷胱甘肽（GSH）荧光探针。当与待测试细胞培养物共同孵育时，它可以轻易进入细胞并形成荧光复合物。单氯二胺-GSH反应由谷胱甘肽-S-转移酶催化，可使用荧光法检测。

单氯二胺又名mBCl，是一种反应前不发荧光，反应后形成荧光复合物的化合物。 荧光可在394/490nm处被检测到。

应用

单氯二胺在荧光谷胱甘肽测定中用作荧光剂。 它被用于检测主要细胞内小分子量的硫醇，这些硫在防御机制中起关键作用。

包装

无底玻璃瓶。内含物装在插入的融合锥内。

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315,H319,H335

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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Birgitta I Eklund et al.
Analytical biochemistry, 309(1), 102-108 (2002-10-17)
A rapid and facile colony assay has been developed for catalytically active enzymes in combinatorial cDNA libraries of mutated glutathione transferases (GST), expressed in Escherichia coli. The basis of the method is the conjugation of glutathione (GSH) with the fluorogenic
A Jos et al.
Toxicology in vitro : an international journal published in association with BIBRA, 23(3), 546-553 (2009-05-16)
Reduced glutathione (GSH) protects cells against injury by oxidative stress and maintains a range of vital functions. In vitro cell cultures have been used as experimental models to study the role of GSH in chemical toxicity in mammals; however, this
Peter Schröder et al.
Environmental science and pollution research international, 14(2), 114-122 (2007-04-26)
Numerous herbicides and xenobiotic organic pollutants are detoxified in plants to glutathione conjugates. Following this enzyme catalyzed reaction, xenobiotic GS-conjugates are thought to be compartmentalized in the vacuole of plant cells. In the present study, evidence is presented from experiments
Yoshihiro Nakano et al.
Bioscience, biotechnology, and biochemistry, 70(7), 1790-1793 (2006-07-25)
Previously we reported the purification of soluble gamma-glutamyltransferases (GGTs) from radish cotyledon. Subcellular fractionation of radish cells revealed that soluble GGT is a vacuolar enzyme. Acivicin, a GGT inhibitor, mediated the in vivo catabolism inhibition of the glutathione S-conjugate generated
Jana Wünschmann et al.
Phytochemistry, 71(1), 54-61 (2009-11-10)
Xenobiotics are widely used as pesticides. The detoxification of xenobiotics frequently involves conjugation to glutathione prior to compartmentalization and catabolism. In plants, degradation of glutathione-S-conjugates is initiated either by aminoterminal or carboxyterminal amino acid cleavage catalyzed by a gamma-glutamyl transpeptidase
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