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质量水平
表单
solid
分子量
15-600 kDa
分析物化学类别
amino acids, peptides, proteins
技术
gel permeation chromatography (GPC): suitable
应用
clinical
food and beverages
pharmaceutical
包装形式
neat
储存温度
−20°C
一般描述
蛋白标准品混合物是校准用标准品，用于测试和监测尺寸排阻色谱（SEC）柱的性能。它是分子量从 15 kDa 到 600 kDa 的标记物的冻干混合物。
组分
甲状腺球蛋白牛 MW ~ 670 000 Da
牛血液中的 γ-球蛋白 MW ~ 150 000 Da
卵白蛋白 MW~ 44 300 Da
核糖核酸酶 A I-A 型 MW ~ 13 700 Da
对氨基苯甲酸 (pABA) MW ~ 137 Da
组分
甲状腺球蛋白牛 MW ~ 670 000 Da
牛血液中的 γ-球蛋白 MW ~ 150 000 Da
卵白蛋白 MW~ 44 300 Da
核糖核酸酶 A I-A 型 MW ~ 13 700 Da
对氨基苯甲酸 (pABA) MW ~ 137 Da
应用
本分析标准用于以下方面：
- 通过不同流速、硅胶颗粒大小和柱孔大小评价粒径排除色谱（SEC）法分离完整蛋白的选择性和分离效率
- 通过粒径排除色谱-高效液相色谱-二极管阵列检测（SEC-HPLC-DAD）同时测定寡聚化和硝化蛋白
- 以牛血清白蛋白（BSA）作为模型蛋白，经 O3 和 NO2 诱导氧化、硝化和寡聚，通过尺寸排除层析法对蛋白质进行分子量分离
- 通过粒径排除色谱（SEC）法估算两种重组蛋白 TNF 荧光传感器（BTN-Kat）和荧光传感器抑制剂（ITN-Kat）的分子量，评估其在体外结合和中和肿瘤坏死因子（TNF）的能力，并进一步作为非侵入性分析的成像标记
相关产品
产品编号
说明
价格
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
Christopher J Kampf et al.
Environmental science & technology, 49(18), 10859-10866 (2015-08-20)
Air pollution is a potential driver for the increasing prevalence of allergic disease, and post-translational modification by air pollutants can enhance the allergenic potential of proteins. Here, the kinetics and mechanism of protein oligomerization upon ozone (O3) exposure were studied
Different Structural Conformers of Monomeric α-Synuclein Identified after Lyophilizing and Freezing.
Amberley D Stephens et al.
Analytical chemistry, 90(11), 6975-6983 (2018-05-12)
Understanding the mechanisms behind amyloid protein aggregation in diseases, such as Parkinson's and Alzheimer's disease, is often hampered by the reproducibility of in vitro assays. Yet, understanding the basic mechanisms of protein misfolding is essential for the development of novel
Thomas Fricke et al.
Viruses, 14(3) (2022-03-27)
Kaposi's sarcoma herpesvirus (KSHV) is associated with a significant disease burden, in particular in Sub-Sahara Africa. A KSHV vaccine would be highly desirable, but the mechanisms underlying neutralizing antibody responses against KSHV remain largely unexplored. The complex made of glycoproteins
Andrew J Bowling et al.
Toxins, 11(5) (2019-05-19)
Vip3A proteins are important for the control of spodopteran pests in crops, including Spodoptera frugiperda (fall armyworm). Native Vip3Ab1 controls S. frugiperda, but it is ineffective against S. eridania (southern armyworm), a major pest of soybean in South America. Recently
Robert C Monsen et al.
Nucleic acids research, 49(3), 1749-1768 (2021-01-21)
Human telomeres contain the repeat DNA sequence 5'-d(TTAGGG), with duplex regions that are several kilobases long terminating in a 3' single-stranded overhang. The structure of the single-stranded overhang is not known with certainty, with disparate models proposed in the literature.
商品
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
体积排阻色谱（SEC）色谱柱和现成的标准品有助于方法开发，并提高蛋白质SEC方法的耐用性。
Characterize mAb monomers, aggregates, and fragments using SEC-UV workflow with Zenix® and Zenix®-C SEC columns.
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