推荐产品
质量水平
形式
solid
分子量
15-600 kDa
分析物化学类别
amino acids, peptides, proteins
技术
gel permeation chromatography (GPC): suitable
应用
clinical
food and beverages
pharmaceutical
格式
neat
储存温度
−20°C
一般描述
蛋白标准品混合物是校准用标准品，用于测试和监测尺寸排阻色谱（SEC）柱的性能。它是分子量从 15 kDa 到 600 kDa 的标记物的冻干混合物。
组分
甲状腺球蛋白牛 MW ~ 670 000 Da
牛血液中的 γ-球蛋白 MW ~ 150 000 Da
卵白蛋白 MW~ 44 300 Da
核糖核酸酶 A I-A 型 MW ~ 13 700 Da
对氨基苯甲酸 (pABA) MW ~ 137 Da
组分
甲状腺球蛋白牛 MW ~ 670 000 Da
牛血液中的 γ-球蛋白 MW ~ 150 000 Da
卵白蛋白 MW~ 44 300 Da
核糖核酸酶 A I-A 型 MW ~ 13 700 Da
对氨基苯甲酸 (pABA) MW ~ 137 Da
应用
本分析标准用于以下方面：
- 通过不同流速、硅胶颗粒大小和柱孔大小评价粒径排除色谱（SEC）法分离完整蛋白的选择性和分离效率
- 通过粒径排除色谱-高效液相色谱-二极管阵列检测（SEC-HPLC-DAD）同时测定寡聚化和硝化蛋白
- 以牛血清白蛋白（BSA）作为模型蛋白，经 O3 和 NO2 诱导氧化、硝化和寡聚，通过尺寸排除层析法对蛋白质进行分子量分离
- 通过粒径排除色谱（SEC）法估算两种重组蛋白 TNF 荧光传感器（BTN-Kat）和荧光传感器抑制剂（ITN-Kat）的分子量，评估其在体外结合和中和肿瘤坏死因子（TNF）的能力，并进一步作为非侵入性分析的成像标记
相关产品
产品编号
说明
价格
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
常规特殊物品
常规特殊物品
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Protein expression and purification, 169, 105587-105587 (2020-02-01)
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The valorization of Atlantic cod (Gadus morhua) frames from a filleting industry was investigated using subcritical water extraction and hydrolysis (SBW) at different temperatures (90, 140, 190 and 250 °C) and 100 bar to obtain extracts rich in proteins, peptides
商品
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
体积排阻色谱（SEC）色谱柱和现成的标准品有助于方法开发，并提高蛋白质SEC方法的耐用性。
Characterize mAb monomers, aggregates, and fragments using SEC-UV workflow with Zenix® and Zenix®-C SEC columns.
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