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Merck
CN
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文件

安全信息

67309

Millipore

Kovac 吲哚试剂

suitable for microbiology

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别名:
对二甲氨基苯甲醛 溶液
经验公式（希尔记法）:
C9H11NO
CAS号:
100-10-7
分子量:
149.19
Beilstein:
4132845
MDL编号:
MFCD00003381
UNSPSC代码:
41171621
PubChem化学物质编号:
329760541
NACRES:
NA.85

Agency

according to ISO 16654:2001

质量水平

200

产品线

BioChemika

保质期

limited shelf life, expiry date on the label

组成

4-(dimethlyamino)benzaldehyde, 50 g/L
hydrochloric acid, 240 g/L
isoamylic alcohol, 710 g/L

技术

microbe id | specific enzyme detection: suitable

应用

agriculture
clinical testing
environmental
food and beverages
microbiology

储存温度

2-8°C

适用性

Escherichia coli
coliforms

SMILES字符串

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChI key

BGNGWHSBYQYVRX-UHFFFAOYSA-N

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相关类别

一般描述

通过混合对二甲氨基苯甲醛、异戊醇和浓盐酸，可以制备Kovac试剂。为了鉴定微生物，利用色氨酸底物生成吲哚是一种实用的诊断工具。吲哚产生是鉴定大肠杆菌的关键试验。孵育后，添加试剂将有助于确定是否已产生吲哚。当与吲哚反应时，试剂变红。

应用

Kovac试剂可用于斑点实验，通过用试剂饱和滤纸来测定吲哚。它也可以用于初始斑点实验以测定吲哚，然后通过分光光度测定法和HPLC-UV/Vis-MS/MS方法进行定量。
在有氧的情况下，某些细菌（如大肠杆菌）能够将色氨酸分解为吲哚和α-氨基丙酸。该试剂可用于检测吲哚并鉴定吲哚阳性和吲哚阴性的微生物。

替代产品

产品编号
说明
价格

警示用语：

Danger

危险分类

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 1

闪点(°F)

109.4 °F

闪点(°C)

43 °C

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

易制毒化学品（3类）
危险化学品

分析证书(COA)

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Rita R. Colwell, R. Grigorova
Methods in Microbiology, 19, 27-27 (1987)
Patrick R Porubsky et al.
Archives of biochemistry and biophysics, 475(1), 14-17 (2008-04-22)
Cytochrome P450 2A13 (CYP2A13) is a lung specific enzyme known to activate the potent tobacco procarcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) into two carcinogenic metabolites. CYP2A13 has been crystallized and X-ray diffraction experiments illuminated the structure of this enzyme, but with an unknown
R W Trepeta et al.
Journal of clinical microbiology, 19(2), 172-174 (1984-02-01)
Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and
N T Yatsimirskaya et al.
Analytical biochemistry, 229(2), 249-255 (1995-08-10)
Yields of colored Schiff base products of the reversible condensation of 6-aminopenicillanic and 7-aminocephalosporanic acids with para-dimethylaminobenzaldehyde in a weakly acid aqueous solution strongly increase in the presence of micelles of sodium dodecyl sulfate. The binding constants of reactants and
Jun-Min Guo et al.
Journal of agricultural and food chemistry, 58(11), 6556-6561 (2010-05-15)
A simple colorimetric method for the differentiation of indoleacetic acid (IAA) and indolebutyric acid (IBA) in plant samples is described. The color change is based upon the reaction between the auxins and p-(dimethylamino)benzaldehyde (PDAB, Ehrlich reagent) following the electrophilic substitution
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