产品名称
南极假丝酵母脂肪酶A，重组 来源于米曲霉, powder, beige, ~2 U/mg
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
recombinant
expressed in Aspergillus oryzae
form
powder
specific activity
~2 U/mg
shelf life
limited shelf life, expiry date on the label
color
beige
storage temp.
2-8°C
Quality Level
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Application
南极假丝酵母脂肪酶A（米曲霉生产的重组蛋白）已用于酶催化的3-乙酰硫己醛水解研究。还用作生物催化剂，检测其对植物固醇和羊脂酸的酯化反应的影响。
Biochem/physiol Actions
南极假丝酵母脂肪酶 A 催化叔醇水解形成甘油和脂肪酸，并显示出对 β-氨基酯的 N-酰化的选择性。
南极假丝酵母脂肪酶A(CALA)是热稳定性丝氨酸水解酶，倾向于催化甘油三酯sn-2位的酯键断裂。对大分子底物和大位阻叔醇具有优异的活性。
General description
南极假丝酵母脂肪酶A(CALA)属于α/β水解酶家族。由Ser184、His366和Asp334残基组成催化三联体。
Other Notes
1个酶活性单位相当于在pH 8.0和40°C条件下，每分钟水解1 μmol油酸所需的酶量（使用甘油三油酸酯作为底物，货号62314）。如上所述，1个酶活性单位相当于在pH 8.0和70°C条件下，使用甘油三丁酸酯作为底物时的约0.15个酶活性单位
特性
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
常规特殊物品
此项目有
Integrated enzymatic production of specific structured lipid and phytosterol ester compositions
Hellner G, et al.
Process. Biochem., 45(8), 1245-1250 (2010)
Anders G Sandström et al.
Protein engineering, design & selection : PEDS, 22(7), 413-420 (2009-06-11)
We herein report the first directed evolution of Candida antarctica lipase A (CalA), employing a combinatorial active-site saturation test (CAST). Wild-type CalA has a modest E-value of 5.1 in kinetic resolution of 4-nitrophenyl 2-methylheptanoate. Enzyme variants were expressed in Pichia
Biotechnological relevance of the lipase A from Candida antarctica
Monteiro RRC, et al.
Catalysis Today, 362, 141-154 (2021)
S.A. Patkar et al.
Indian Journal of Chemistry, 76-76 (1993)
Hidehiko Wakabayashi et al.
Journal of agricultural and food chemistry, 51(15), 4349-4355 (2003-07-10)
The enantioselectivity of the generation of 3-mercaptohexanal and 3-mercaptohexanol, two potent sulfur-containing aroma compounds, by lipase-catalyzed hydrolysis of the corresponding 3-acetylthioesters was investigated. The stereochemical course of the kinetic resolutions was followed by capillary gas chromatography using modified cyclodextrins as
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