等级
for molecular biology
质量水平
产品线
BioUltra
形式
liquid
质量
2× concentrate
组成
dextrose, 2.0 g/L
HEPES, 10 g/L
KCl, 0.74 g/L
NaCl, 16 g/L
Na2HPO4.2H2O, 0.27 g/L
浓度
2 ×
杂质
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
折射率
n20/D 1.339
pH值(酸碱度)
7.2 (25 °C)
痕量阳离子
Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg
λ
neat
紫外吸收
λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
SMILES字符串
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
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一般描述
HEPES 缓冲盐水是组织培养缓冲液,其用于维持细胞培养物的 pH。它是水溶性的,不依赖大气。HEPES 在氯化钠溶液中破坏镁。
应用
HEPES 缓冲盐水已被使用:
- 用于制备质粒 DNA
- 作为制备用于 DNA 构建载体的转染主混合物的试剂
- 开发用于细胞渗漏测定的水凝胶前体
组分
浓度(溶于 H2O):10g/L HEPES;16g/L NaCl;0.74g/L KCl;0.27g/L Na2HPO4.2H2O;2.0g/L 右旋糖
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
Attachment of microstructures to single bacteria by two-photon patterning of a protein based hydrogel
Biomedical Physics & Engineering Express, 4(3), 035004-035004 (2018)
Molecular Cloning: A Laboratory Manual, B11 (1989)
Calcium phosphate transfection.
Current Protocols in Cell Biology, 20, 20-20 (2003)
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (2015)
A new approach to neural cell culture for long-term studies
Journal of Neuroscience Methods, 110(1-2), 17-24 (2001)
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