一般描述
按照 C.H. Fiske 和 Y. Subbarow(J.Biol.Chem. 66,375 (1925))的方法配制的 1-氨基-2-萘酚-4-磺酸、亚硫酸钠和亚硫酸氢钠
应用
The Fiske-Subbarow assay is a colorimetric assay designed to measure levels of inorganic phosphate in biological samples. Fiske-Subbarow reducer reagent is used as the reducer component of the Fiske-Subbarow assay.
警示用语:
Danger
危险声明
危险分类
Acute Tox. 4 Oral - Eye Dam. 1
补充剂危害
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
危险化学品
Analytical biochemistry, 145(2), 230-234 (1985-03-01)
A new colorimetric procedure is described for inorganic phosphate determination using the color reaction between inorganic phosphate and acidified ammonium molybdate in the presence of Triton X-100. The method is simple and specific, and produces results comparable with those of
Analytical biochemistry, 348(1), 57-63 (2005-11-18)
We describe the development of a rapid colorimetric assay for soluble guanylate cyclase (sGC) activity adapted for a 96-well microplate. The assay greatly decreases the analysis time and cost over traditional methodologies based on radio- and immunoassays and high-performance liquid
BioTechniques, 25(4), 608-612 (1998-10-30)
Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method
The colorimetric determination of phosphorus
The Journal of Biological Chemistry, 66, 375-375 (1925)
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