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410217

Sigma-Aldrich

2′,7′-二氯荧光素

ACS reagent

别名:

二氯荧光素；荧光素27

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About This Item

经验公式（希尔记法）:
C20H10Cl2O5
CAS号:
76-54-0
分子量:
401.20
Beilstein:
58009
EC 号:
200-968-6
MDL编号:
MFCD00005047
UNSPSC代码:
12171500
PubChem化学物质编号:
24865700
NACRES:
NA.47

等级

ACS reagent

质量水平

200

表单

powder

技术

titration: suitable

颜色

orange to red-brown, powder

mp

280 °C (dec.) (lit.)

溶解性

alcohol: passes test

密度

0.790 g/cm3

λmax

509 nm

适用性

passes test for adsorption indicator

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

VFNKZQNIXUFLBC-UHFFFAOYSA-N

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相关类别

一般描述

2′,7′-二氢荧光素是一种氧化敏感荧光探针。

应用

2′,7′-二氯荧光素已用于测量细胞中ROS（活性氧）的形成。 它已被用作荧光底物，以研究唾液样本及颌下腺提取物的过氧化物酶的活性。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCL8813
BCCK3080
BCCH5169
BCCF7954
BCBG1058V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What can be used to make a solution of 2´,7´-dichlorofluorescein?

    2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.

  6. In what types of applications can 2´,7´-Dichlorofluorescein be used?

    Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC.  See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a  fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).

  7. What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?

    2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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