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一般描述
达布酰氯(4-二甲氨基偶氮苯-4')-磺酰氯)是一种发色团标记试剂,在 HPLC 中用于衍生氨基酸。与所有氨基酸按顺序自由反应形成 absyl 氨基酸,具有光稳定性,且可在薄层色谱板上显示。
应用
用于氨基酸的定性和定量鉴定以及 N-末端氨基酸的测定。用于 HPLC 法测定伯胺和仲胺。
警示用语:
Danger
危险声明
危险分类
Skin Corr. 1B
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
Chromophoric labeling of amino acids with 4-dimethylaminoazobenzene-4'-sulfonyl chloride.
Analytical chemistry, 47(9), 1634-1638 (1975-08-01)
Biochemistry, 32(14), 3623-3628 (1993-04-13)
The structure of the gene 5 protein of filamentous bacteriophage Pf1 and its interaction with viral DNA have been investigated by a series of limited proteolysis experiments. The ability of purified proteolytic fragments of the Pf1 gene 5 protein to
Analytical biochemistry, 226(1), 1-9 (1995-03-20)
We have developed a new method for the quantification of phosphoserine, phosphothreonine, and phosphotyrosine as dabsyl derivatives in acid-hydrolyzed extracts of 32P-labeled A431 cells. In the first step the phosphamino acids are concentrated using a disposable anion-exchange column. Subsequently, the
Journal of combinatorial chemistry, 3(5), 410-420 (2001-09-11)
The presence of dansyl or dabsyl chromogenic moieties in a solid-phase analytical construct, an assembly of linkers/spacers/sensitizers for improving analytical characterization, allows the accurate estimation of products from solid-phase synthesis by UV detection during liquid chromatography-mass spectrometry analysis in the
Journal of pharmaceutical and biomedical analysis, 14(8-10), 1063-1067 (1996-06-01)
A method has been developed for the analysis of phosphoserine, phosphothreonine and phosphotyrosine in 32P-phosphoprotein hydrolysates. The hydrolysates are treated with dabsyl reagent (28.8 mM) for 10 min at 70 degrees C. After a clean-up using a disposable C18 column
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