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安全信息

37008

Sigma-Aldrich

毛地黄皂苷

~50% (TLC)

别名:

Digitin

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About This Item

经验公式（希尔记法）:
C56H92O29
CAS号:
11024-24-1
分子量:
1229.31
Beilstein:
78654
EC 号:
234-255-6
MDL编号:
MFCD00077729
UNSPSC代码:
12352200

描述

non-ionic

旋光性

[α]20/D −54.5±3°, c = 1% in methanol (dry matter)

分子量

micellar avg mol wt 70,000

浓度

~50% (TLC)

聚集数

60

CMC

<0.5 mM (20-25°C)

mp

230-240 °C (dec.) (lit.)

溶解性

ethanol: 10 mg/mL, clear, almost colorless (hot)

SMILES字符串

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])[C@@H](O)[C@@]1([H])[C@@](CC[C@]5([H])[C@@]6(C[C@@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@H]7O[C@@](O[C@H](CO)[C@@H](O)[C@@H]8O[C@@](OC[C@@H](O)[C@@H]9O)([H])[C@@H]9O)([H])[C@@H]8O[C@@](O[C@H](CO)[C@H

InChI

1S/C56H92O29/c1-19-7-10-56(75-17-19)20(2)31-45(85-56)37(67)32-22-6-5-21-11-26(24(61)12-55(21,4)23(22)8-9-54(31,32)3)76-50-42(72)39(69)44(30(16-60)80-50)81-53-48(47(36(66)29(15-59)79-53)83-49-40(70)33(63)25(62)18-74-49)84-52-43(73)46(35(65)28(14-58)78-52)82-51-41(71)38(68)34(64)27(13-57)77-51/h19-53,57-73H,5-18H2,1-4H3/t19-,20+,21+,22-,23+,24-,25-,26-,27-,28-,29-,30-,31+,32-,33+,34-,35+,36-,37+,38+,39-,40-,41-,42-,43-,44+,45-,46+,47+,48-,49+,50-,51+,52+,53+,54-,55+,56-/m1/s1

InChI key

UVYVLBIGDKGWPX-KUAJCENISA-N

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应用

suitable for determination of cholesterol

包装

Bottomless glass bottle. Contents are inside inserted fused cone.

其他说明

Permeabilizes plasma membranes of eukaryotic cells by complexing with membrane cholesterol and other unconjugated β-hydroxysterols. Use of digitonin-permeabilized adipocytes for cAMP studies; Selective precipitation of cholesterol; Solubilization of membrane proteins; Opioid receptor proteins.

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T J Scallen et al.
The Journal of cell biology, 40(3), 802-813 (1969-03-01)
A major methodological problem in the intracellular localization of cholesterol is the nearly complete extraction of sterols during routine dehydration and embedding procedures for electron microscopy. Cholesterol digitonide (a sterol complex with digitonin), however, is qualitatively insoluble in these solvents.
C D Demoliou-Mason et al.
FEBS letters, 170(2), 378-382 (1984-05-21)
The binding sites for opiates (agonist and antagonist) and opioid peptides can be solubilized from rat brain membranes with digitonin in the presence of Mg2+ (10 mM). High affinity and high capacity binding to the soluble delta, mu, and kappa
Solubilization of functional membrane proteins.
L M Hjelmeland et al.
Methods in enzymology, 104, 305-318 (1984-01-01)
Use of digitonin-permeabilized adipocytes for cAMP studies.
R A Mooney
Methods in enzymology, 159, 193-202 (1988-01-01)

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