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Merck
CN

30911

用于 RNA 分离的变性溶液

BioUltra, Molecular Biology

别名:

RNA 分离,变性

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
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grade

Molecular Biology

product line

BioUltra

composition

guanidine thiocyanate, 4 M , Na-citrate, 25 mM , sarcosyl, 0.5%

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.433

pH

5.5-7.0

anion traces

chloride (Cl-): ≤500 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤2 mg/kg, As: ≤0.1 mg/kg, Ba: ≤2 mg/kg, Bi: ≤2 mg/kg, Ca: ≤10 mg/kg, Cd: ≤2 mg/kg, Co: ≤2 mg/kg, Cr: ≤2 mg/kg, Cu: ≤2 mg/kg, Fe: ≤2 mg/kg, K: ≤100 mg/kg, Li: ≤2 mg/kg, Mg: ≤2 mg/kg, Mn: ≤2 mg/kg, Mo: ≤2 mg/kg, Ni: ≤2 mg/kg, Pb: ≤2 mg/kg, Sr: ≤2 mg/kg, Zn: ≤2 mg/kg

λ

neat

UV absorption

λ: 280 nm Amax: 1

Quality Level

Application

Denaturation solution for the isolation of RNA is a guanidine thiocyanate, sarcosyl based protein denaturization solution useful for the purification of mRNA.

Other Notes

通过酸-硫氰酸胍-苯酚-氯仿萃取进行 RNA 分离的单步方法

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

存储类别

8A - Combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Y L Tsai et al.
Applied and environmental microbiology, 57(3), 765-768 (1991-03-01)
A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is

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