推荐产品
等级
for molecular biology
质量水平
产品线
BioUltra
组成
guanidine thiocyanate, 4 M
Na-citrate, 25 mM
sarcosyl, 0.5%
杂质
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
折射率
n20/D 1.433
pH值（酸碱度）
5.5-7.0
痕量阴离子
chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg
痕量阳离子
Al: ≤2 mg/kg
As: ≤0.1 mg/kg
Ba: ≤2 mg/kg
Bi: ≤2 mg/kg
Ca: ≤10 mg/kg
Cd: ≤2 mg/kg
Co: ≤2 mg/kg
Cr: ≤2 mg/kg
Cu: ≤2 mg/kg
Fe: ≤2 mg/kg
K: ≤100 mg/kg
Li: ≤2 mg/kg
Mg: ≤2 mg/kg
Mn: ≤2 mg/kg
Mo: ≤2 mg/kg
Ni: ≤2 mg/kg
Pb: ≤2 mg/kg
Sr: ≤2 mg/kg
Zn: ≤2 mg/kg
λ
neat
紫外吸收
λ: 280 nm Amax: 1
应用
Denaturation solution for the isolation of RNA is a guanidine thiocyanate, sarcosyl based protein denaturization solution useful for the purification of mRNA.
其他说明
通过酸-硫氰酸胍-苯酚-氯仿萃取进行 RNA 分离的单步方法
警示用语：
Danger
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
补充剂危害
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Y L Tsai et al.
Applied and environmental microbiology, 57(3), 765-768 (1991-03-01)
A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is
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