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Merck
CN

211842

Brilliant Black BN

Dye content 60 %

别名:

Food Black 1

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关于此项目

经验公式（希尔记法）:
C28H17N5Na4O14S4
化学文摘社编号:
2519-30-4
分子量:
867.68
PubChem Substance ID:
24852710
UNSPSC Code:
12171500
Colour Index Number:
28440
NACRES:
NA.47
EC Number:
219-746-5
MDL number:
MFCD00078980

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产品名称

Brilliant Black BN, Dye content 60 %

InChI key

GMMAPXRGRVJYJY-OXYXOBGMSA-J

InChI

1S/C28H21N5O14S4.4Na/c1-14(34)29-23-10-11-24(50(42,43)44)20-13-25(51(45,46)47)27(28(35)26(20)23)33-32-22-9-8-21(18-7-6-17(12-19(18)22)49(39,40)41)31-30-15-2-4-16(5-3-15)48(36,37)38;;;;/h2-13,35H,1H3,(H,29,34)(H,36,37,38)(H,39,40,41)(H,42,43,44)(H,45,46,47);;;;/q;4*+1/p-4/b31-30-,33-32+;;;;

SMILES string

[Na+].[Na+].[Na+].[Na+].CC(=O)Nc1ccc(c2cc(c(\N=N\c3ccc(\N=N/c4ccc(cc4)S([O-])(=O)=O)c5ccc(cc35)S([O-])(=O)=O)c(O)c12)S([O-])(=O)=O)S([O-])(=O)=O

form

powder

composition

Dye content, 60%

λmax

407 nm (2nd)
570 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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相关类别

Application

Brilliant Black BN has been used:
  • in micronucleus and comet assays to evaluate its genotoxic effect.
  • as a quenching agent in calcium mobilization assay.
  • as a redox indicator in microbiological inhibition bioassay using Bacillus megaterium to detect tetracycline and macrolides in milk.

Biochem/physiol Actions

[4-acetamido-5-hydroxy-6-[7-sulfonato-4-(4-sulfonatophenylazo)-1-naphtylazo], also known as food black 1, belongs to the group of azo dyes. Brilliant Black BN is widely used as a food dye and pharmaceutical excipient. In addition, it also acts as an allosteric modulator of the adenosine A1 and A3 receptors and is generally used within calcium mobilization assays to reduce extracellular fluorescence.

存储类别

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SHBT2811
SHBQ6363
SHBN6839
SHBL4641
MKCC1198

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Novel bioassay using Bacillus megaterium to detect tetracycline in milk
Tumini M
Revista Argentina de microbiologia, 48, 143-146 (2016)
Antagonist selective modulation of adenosine A1 and A3 receptor pharmacology by the food dye Brilliant Black BN: evidence for allosteric interactions.
May LT
Molecular Pharmacology, 77, 678-686 (2010)
The evaluation of the genotoxicity of two commonly used food colors: Quinoline Yellow (E 104) and Brilliant Black BN (E 151).
Cellular & Molecular Biology Letters, 9, 107-122 (2004)
Calcium Mobilisation Assay in Response to Chemokine Stimulation
Cronshaw DG
Bio-protocol, 2 (2012)
Microbiological Method using Bacillus megaterium
with Fusidic Acid for Detection of Macrolides in Milk
Tumin M
Czech Journal of Food Sciences, 34, 9-15 (2016)
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