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Merck
CN

21022

Supelco

三氟乙酸铯

BioUltra, for molecular biology, ≥99.0% (NT)

别名:

CsTFA, 三氟乙酸 铯盐

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About This Item

线性分子式:
CF3COOCs
CAS号:
分子量:
245.92
Beilstein:
4161420
MDL编号:
UNSPSC代码:
12000000

等级

for molecular biology

产品线

BioUltra

方案

≥99.0% (NT)

杂质

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH值(酸碱度)

9.0-10.5 (25 °C, 2 M in H2O)

mp

114-116 °C (lit.)

溶解性

H2O: 2 M at 20 °C, clear, colorless

痕量阴离子

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤10 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

2 M in H2O

紫外吸收

λ: 260 nm Amax: 0.09
λ: 280 nm Amax: 0.04

SMILES字符串

[Cs+].[O-]C(=O)C(F)(F)F

InChI

1S/C2HF3O2.Cs/c3-2(4,5)1(6)7;/h(H,6,7);/q;+1/p-1

InChI key

RJYSYRSELCQCSO-UHFFFAOYSA-M

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其他说明

Medium in density gradient centrifugation for the separation of DNA, RNA and protein, and for plasmid isolation; Isolation of mRNA prior to the construction of full-length cDNA libraries

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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P E Mirkes
Analytical biochemistry, 148(2), 376-383 (1985-08-01)
A procedure for the simultaneous banding of cellular DNA, RNA, and protein by centrifugation in cesium trifluoroacetate (CsTFA) gradients is described. Starting with homogenates of Day 11 rat embryos, this procedure was used to separate total DNA, RNA, and protein.
K Andersson et al.
Plasmid, 13(1), 78-80 (1985-01-01)
Plasmids extracted from bacterial cells by alkaline extraction can easily be isolated from linear DNA by isopycnic centrifugation in CsTFA. This is a fast and simple method which circumvents the use of the intercalating dye, ethidium bromide, and consequently the
D S Zarlenga et al.
Analytical biochemistry, 162(2), 569-574 (1987-05-01)
A rapid method for simultaneously banding preparative amounts of RNA and DNA from Trichinella spiralis muscle larvae by isopycnic centrifugation in cesium trifluoroacetate (CsTFA) is described. Larvae were homogenized in guanidinium isothiocyanate and the DNA, RNA, glycogen, and denatured protein
High-efficiency cloning of full-length cDNA; construction and screening of cDNA expression libraries for mammalian cells.
H Okayama et al.
Methods in enzymology, 154, 3-28 (1987-01-01)

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