等级
for TLC derivatization
质量水平
形式
liquid
技术
thin layer chromatography (TLC): suitable
杂质
1-butanol
acetic acid
密度
0.823 g/mL at 20 °C
一般描述
Ninhydrin reagent is mostly used for the detection of amino acids. It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.0 or higher. At an acid pH of 0.3, the reaction of ninhydrin with ornithinine and proline results in a red color product. Ninhydrin reagent can stay stable at room temperature in the absence of nitrogen atmosphere for upto 1 month in the ethylene glycol-sodium acetate solvent system.
应用
1 g ninhydrin dissolved in 475 mL 1-butanol and 25 mL acetic acid
Ninhydrin reagent may be used for the quantitative determination of total protein content in tissue hydrolysates.
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system, Respiratory system
WGK
WGK 2
闪点(°F)
93.2 °F
闪点(°C)
34 °C
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
法规信息
新产品
M K Gaitonde
The Biochemical journal, 104(2), 627-633 (1967-08-01)
1. An acid ninhydrin reagent was found to react specifically in forming a pink product (E(max.) 560mmu) with cysteine. 2. The method was highly sensitive for the determination of cysteine (in 28.0x10(3)). Homocysteine, glutathione, proline, ornithine and other naturally occurring
Thin Layer Chromatography in Phytochemistry.
Hajnos WM
Science, 312-312 (2008)
B Starcher
Analytical biochemistry, 292(1), 125-129 (2001-04-26)
Quantitation of small tissue samples for total protein content is essential for many biochemical analyses. In this study a ninhydrin method for measuring the total protein content of tissue hydrolysates is presented. The ninhydrin reagent is stable at room temperature
Sylvia Kristyanto et al.
Journal of microbiology (Seoul, Korea), 56(5), 317-323 (2018-05-04)
A novel, aerobic, Gram-stain-negative, non-motile, non-spore forming, rod-shaped bacterium, designated strain Dol 15-39T, was isolated from a seawater sample near Geoje Island in the South Sea, Republic of Korea. The strain was found to be oxidase-negative and catalase-positive. The isolate
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