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生物来源
synthetic
质量水平
方案
≥99.0% (TLC)
表单
powder
分子量
Mr ~70000
标记范围
5-25 mol/mol substitution (biotin)
颜色
colorless to white
溶解性
H2O: 50 mg/mL, slightly hazy, faintly yellow
应用
detection
储存温度
2-8°C
一般描述
Biotin/vitamin B7/vitamin H is a water-soluble B complex vitamin. It consists of an uredo ring fused by a tetrahydrothiophene ring. Biotin is readily available in several foods, like whole grains, nuts, legumes, unpolished rice and egg yolk. The normal gut flora also produces this vitamin.
应用
Biotin, immobilized on dextran has been used in in vivo labeling of the lectin structures of vascular endothelium to demonstrate the functionality of CD31+ vessels.
Dextrans are used in many applications as volume extenders, stabilizers, matrix components, binding platforms, lubricants and physical structure components. Biotinylated dextran may be used to capture or detect avidin/strepavidin conjugated molecules.
生化/生理作用
Biotin acts as an important cofactor for carboxylase enzymes in various metabolic pathways. It is implicated in keratin synthesis and hence is used as a supplement for skin, hair and nail growth. Biotin functions as a coenzyme in the metabolism of fats, fatty acids and amino acids such as isoleucine and valine. It is involved in gluconeogenesis and citric acid cycle. Biotin is essential for cell growth.It assists the transfer of carbon dioxide and also maintains a steady blood sugar level.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
N Rajakumar et al.
Brain research, 607(1-2), 47-53 (1993-04-02)
A powerful and versatile axonal tracing method using biotinylated dextran, a novel analogue of biotin, is described. Pressure injection of varying volumes of 5% biotinylated dextran into various parts of the brain and spinal cord resulted in Golgi-like retrograde labeling
Ying-Jen Chen et al.
Small (Weinheim an der Bergstrasse, Germany), 14(22), e1703705-e1703705 (2018-05-03)
The noninvasive monitoring of protein secretion of cells responding to drug treatment is an effective and essential tool in latest drug development and for cytotoxicity assays. In this work, a surface functionalization method is demonstrated for specific detection of protein
N Emans et al.
Biophysical journal, 69(2), 716-728 (1995-08-01)
A fluorescence assay of in vivo endosome fusion was developed and applied to define the kinetics of endosome fusion in baby hamster kidney (BHK) fibroblasts. The assay is based on an approximately 10-fold enhancement of the green fluorescence of BODIPY-avidin
Revascularization of the graft in obliterative bronchiolitis after heterotopic tracheal transplantation
Nemska S, et al.
Physiological Reports, 4(4) (2016)
Vitamin H and their role in ruminant: A Review
Pour HA, et al.
Annals of Biological Research, 3(4), 1929-1933 (2012)
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