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Merck
CN

114413

Sigma-Aldrich

溴百里酚蓝

ACS reagent, Dye content 95 %

别名:

3′,3″-二溴百里酚磺酞

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About This Item

经验公式(希尔记法):
C27H28Br2O5S
CAS号:
分子量:
624.38
Beilstein:
373934
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

等级

ACS reagent

质量水平

表单

powder

组成

Dye content, 95%

颜色

pink-purple

visual transition interval

6.0-7.6, yellow to blue(passes test)

mp

200-202 °C ((392 - 396 °F) - lit.)
200-202 °C (lit.)

λmax

420 nm

ε (消光系数)

≥17000 at 419-425 nm in methanol
≥8000 at 326-332 nm in methanol
≥9000 at 279-285 nm in methanol

储存温度

room temp

SMILES字符串

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3

InChI key

NUHCTOLBWMJMLX-UHFFFAOYSA-N

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相关类别

一般描述

溴百里酚蓝,又称为二溴百里酚磺酞,是酸性水溶液中的磺化羟醌。在碱性溶液中,这种染料以中等大小的离子形式存在。它也被称为溴百里酚蓝和二溴百里酚磺酞。它是一种pH值敏感的染料,可从黄色变成蓝色。

应用

溴百里酚蓝染料具有精确的中性颜色过渡,因此可作为 pH 指示剂。 其他应用包括:
  • 作为重要染色剂,用于在显微镜下跟踪淋巴液运动并界定细胞壁或细胞核
  • 用于展示植物根内的真菌菌丝
  • 评估单粒水稻的可溶性酸度
  • 用于琼脂凝胶培养基中蜡样芽孢杆菌的计数
  • 作为几种细菌生长和检测底物的组分,如胱氨酸乳糖电解质缺乏(CLED/BROLACIN);丙酮酸多粘菌素蛋黄甘露醇溴百里酚蓝(PEMBA)和麦康基琼脂。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Andrea Pastore et al.
Talanta, 219, 121251-121251 (2020-09-06)
A pH colorimetric sensor array (CSA) with fast response time (<1 min) using only one acid-base indicator, Bromothymol Blue (BB), was prepared and characterized by modulating the amount, C, of the surfactant Hexadecyltrimethylammonium p-toluenesulfonate between 0 and 0.3725 gCTApTs/gprecursor with a
Reza Amin et al.
Micromachines, 11(6) (2020-07-01)
To transform from reactive to proactive healthcare, there is an increasing need for low-cost and portable assays to continuously perform health measurements. The paper-based analytical devices could be a potential fit for this need. To miniaturize the multiplex paper-based microfluidic
Hisataka Maruyama et al.
Lab on a chip, 8(2), 346-351 (2008-01-31)
This paper demonstrates local pH measurement in a microchip using a pH-sensing gel-microbead. To achieve this, the gel-microbead made of a hydrophilic photo-crosslinkable resin was functionalized with the pH indicator bromothymol blue (BTB). The primary constituent of this photo-crosslinkable resin
Olga A Zaporozhets et al.
Analytica chimica acta, 597(1), 171-177 (2007-07-31)
Solid-phase spectrophotometric and visual test-methods of fluoride and oxalate determination are proposed. The methods are based on the competitive reactions of ZrOCl2 with methylthymol blue immobilized on silica gel and fluoride or oxalate in solution. Absorbance of the solid-phase reagent
Joseph A Price
Journal of biochemical and biophysical methods, 70(3), 441-444 (2006-12-16)
Phospholipase A(2) is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is

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