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Merck
CN

114375

Sigma-Aldrich

溴甲酚紫

BioReagent, suitable for indicator, Dye content 90 %

别名:

5,5′-二溴-邻甲酚磺酰酞, 溴甲酚紫磺酸内酯

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About This Item

经验公式(希尔记法):
C21H16Br2O5S
CAS号:
分子量:
540.22
Beilstein:
359618
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

产品线

BioReagent

质量水平

检测方案

≥89.0% (HPLC)

形式

powder

组成

Dye content, 90%

技术

titration: suitable

pH值(酸碱度)

5.2-6.8, yellow to purple

visual transition interval

5.2-6.8, yellow to blue

mp

240 °C (dec.) (lit.)

溶解性

ethanol: 1 mg/mL

λmax

419 nm

ε (消光系数)

≥22000 at 418-424 nm in methanol

适用性

suitable for indicator

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

InChI key

ABIUHPWEYMSGSR-UHFFFAOYSA-N

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应用

溴甲酚紫已用于染色 酿酒酵母
血清-白蛋白的分光光度测定

生化/生理作用

溴甲酚紫是一种 pH 指示剂,可将颜色从黄色(低至 pH 5.2)变为紫色(高于 pH 6.8)。主要用作酵母细胞中的荧光染料。有助于细胞膜损伤细胞的死细胞计数。细胞膜正常的细胞不被染色,而受损的细胞则为蓝灰色重影。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


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Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
Kurzweilova H and Sigler K
Yeast, 9, 1207-1211 (1993)
Yeast colonies synchronise their growth and development.
Palkova Z and Forstova J
Journal of Cell Science, 113, 1923-1928 (2000)
ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.
Kulakovskaya TV, et al.
FEMS Yeast Research, 3, 401-404 (2003)
André Klauss et al.
Photosynthesis research, 102(2-3), 499-509 (2009-03-31)
Determination of thermodynamic parameters of water oxidation at the photosystem II (PSII) manganese complex is a major challenge. Photothermal beam deflection (PBD) spectroscopy determines enthalpy changes (ΔH) and apparent volume changes which are coupled with electron transfer in the S-state
M Arab Chamjangali et al.
Journal of hazardous materials, 166(2-3), 701-705 (2009-01-13)
A simple, selective, and sensitive kinetic spectrophotometric method is described for the determination of trace amounts of phenylhydrazine, which is based on its inhibition effect on the reaction between meta cresol purple (MCP) and periodate in the presence of bromide

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