生物来源
bacterial (Thermus aquaticus BM)
质量水平
重组
expressed in E. coli
描述
5 U/βl
Number of Reactions:
If 1.25 U are used per 50 μL reaction, Taq DNA Polymerase, dNTPack is designed for approximately sufficient for number of reactions, mentioned in the usage.
形式
liquid
用途
sufficient for 2,000 reactions (04728904001)
sufficient for 4,000 reactions (04728858001)
sufficient for 400 reactions (04728874001)
sufficient for 80 reactions (04728866001)
sufficient for 800 reactions (04728882001)
分子量
95 kDa
特点
dNTPs included
hotstart: no
包装
pkg of 1,000 U (04728882001 [4 x 250 U])
pkg of 2,500 U (04728904001 [10 x 250 U])
pkg of 5,000 U (04728858001 [20 x 250 U])
pkg of 100 U (04728866001)
pkg of 500 U (04728874001 [2 x 250 U])
制造商/商品名称
Roche
浓度
0.025 units/reaction
参数
72 °C optimum reaction temp.
技术
PCR: suitable
颜色
colorless
输入
purified DNA
最佳pH
~9.0 (20 °C)
溶解性
water: soluble
适用性
suitable for PCR and automated sequencing reactions
UniProt登记号
应用
genomic analysis
life science and biopharma
异质活性
endonucleases, none detected
exonuclease, none detected
nicking activitities, none detected
储存温度
−20°C
一般描述
Contents
Taq DNA Polymerase, 5 U/μl
PCR Buffer, 10x concentrated, with MgCl2
PCR Nucleotide Mix
For maximum convenience, select the ready-to-use 2x concentrated PCR Master.
Taq DNA Polymerase, 5 U/μl
PCR Buffer, 10x concentrated, with MgCl2
PCR Nucleotide Mix
For maximum convenience, select the ready-to-use 2x concentrated PCR Master.
Each dNTPack contains 10 mM additive-free sodium salt nucleotides as a ready-to-use mix.
Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.
应用
Achieve the most consistent results in simple, routine PCR by using Roche Applied Science Taq DNA Polymerase, which is held to our rigorous purity and quality standards. The Taq DNA Polymerase, dNTPack, combines our standard preparation (5 U/μl) of recombinant Taq DNA Polymerase with our convenient, ready-to-use PCR Nucleotide Mix. In all other respects, this preparation is identical to our standard preparation and can be used for:
- PCR
- RT-PCR
- Other primer-extension reactions, such as sequencing and labeling
特点和优势
- Reliable reproducible results: High lot-to-lot consistency.
- No need to test each lot: Taq DNA Polymerase is rigorously tested.
- Prevent PCR carryover: dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.
包装
1 kit containing 3 components
质量
Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.
单位定义
One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 5 U/μl
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 5 U/μl
储存及稳定性
Containers which are opened must be carefully resealed and
kept upright to prevent leakage
kept upright to prevent leakage
其他说明
仅用于生命科学研究。不可用于诊断。
Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.
法律信息
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
仅试剂盒组分
产品编号
说明
- Taq DNA Polymerase 5 U/μl
- PCR Buffer with MgCl<sub>2</sub> 10x concentrated
- PCR Nucleotide Mix
危险声明
预防措施声明
危险分类
Aquatic Chronic 3
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
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