产品名称
链霉亲和素，重组, liquid, ≥17 units/mg protein, pH range 5.0-9.0
biological source
Streptomyces sp. ( Streptomyces avidinii)
recombinant
expressed in E. coli
form
liquid
specific activity
≥17 units/mg protein
mol wt
~52 kDa
packaging
pkg of 1 mg (11721666001)
pkg of 5 mg (11721674001)
manufacturer/tradename
Roche
concentration
70-90%
technique(s)
protein array: suitable
protein purification: suitable
impurities
6-7% water
pH range
5.0-9.0
pI
7.4-7.7 (two main bands)
solubility
water: 10 mg/L (mA405=100)
suitability
suitable for immunological tests
UniProt accession no.
shipped in
cooler
storage temp.
-10 to -25°C
Quality Level
Physical form
白色冻干粉；在 20 mM 磷酸钾 pH 6.5 溶液中冻干
蛋白： 0.7±0.1 mg 蛋白冻干品 (A 282 = 3.1)
KD: 10-15M
蛋白： 0.7±0.1 mg 蛋白冻干品 (A 282 = 3.1)
KD: 10-15M
Application
使用重组链霉亲和素包被固相（，例如 、微孔板、微球 等 ）以及通用免疫学检测系统（链霉亲和素/生物素系统）中的生物素标记半抗原和抗体。
General description
链霉亲和素是一种多功能蛋白质，是从 亲和素 链霉菌中天然产生的。它对生物素表现出很强的亲和性，它们之间能形成一种非共价但很牢固的结合作用。这一独特的性能使链霉亲和素成为多种生物检测系统的理想选择，包括蛋白质印记、酶联免疫吸附测定（ELISA）和免疫组织化学。
Other Notes
一个单元结合 1μg pH 8.9 的 D-生物素。
仅用于生命科学研究。不可用于诊断。
链霉亲和素是一种分子量约为 53kd 的四聚体，由约 13kd 的四个亚基组成，每个亚基含有单个生物素结合位点.
每个亚基有 6 个酪氨酸残基。这种蛋白质不含碳水化合物。
每个亚基有 6 个酪氨酸残基。这种蛋白质不含碳水化合物。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
Automated combinatorial process for nanofabrication of structures using bioderivatized nanoparticles.
Dehlinger D, et al.
Journal of the Association for Laboratory Automation, 5, 267-276 (2007)
Production of Recombinant Streptavidin and Optimization of Refolding Conditions for Recovery of Biological Activity
Jouybari RM et al.
Reports of Biochemistry and Molecular Biology, 6(2), 178-185 (2018)
Michael Weber et al.
Nature photonics, 15(5), 361-366 (2021-05-07)
We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point
John E G McCarthy et al.
Methods in enzymology, 430, 247-264 (2007-10-05)
A growing number of biophysical techniques use immobilized reactants for the quantitative study of macromolecular reactions. Examples of such approaches include surface plasmon resonance, atomic force microscopy, total reflection fluorescence microscopy, and others. Some of these methods have already been
Michael Weber et al.
Nature biotechnology, 41(4), 569-576 (2022-11-08)
Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the
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