推荐产品
生物来源
rat
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
3F10, monoclonal
表单
lyophilized
包装
pkg of 50 μg (11867423001)
pkg of 500 μg (11867431001)
制造商/商品名称
Roche
同位素/亚型
IgG1
表位序列
YPYDVPDYA
储存温度
2-8°C
一般描述
抗-HA高亲和是一种HA-肽链（克隆3F10）的单克隆抗体。抗-HA高亲和识别HA肽链序列（YPYDVPDYA），这一序列来自人类流感血凝素蛋白。即使HA肽链表位通过表位标记，被引入不相关的重组蛋白，该抗体依然可以识别抗原决定簇。
特异性
抗-HA高亲和识别9-氨基酸序列（YPYDVPDYA），这一序列来自人类流感血凝素（HA）蛋白。该表位同样识别融合蛋白，无论它的位置在哪里（N端，C端或中间）。
免疫原
98-106氨基酸来自人类流感病毒血凝素蛋白
应用
使用抗-HA高亲和用于检测天然流感血凝素蛋白和含有HA表位的重组蛋白，可用方法有：
- 斑点印迹法
- ELISA
- 免疫细胞化学
- 免疫沉淀
- Western印迹法
质量
Western印迹法功能测试。
制备说明
工作浓度：偶联物工作浓度取决于用途和底物。
以下浓度可用作参考：
以下浓度可用作参考：
- ELISA：用于检测100ng/ml；用于涂层1至5μg/ml
- 免疫沉淀：0.5至5 μg/ml
- 蛋白质免疫印迹：50到200 ng/ml
重悬
加入0.5ml(11 867 423 001; 50 μg)或2.5ml(11 867 431 001; 500 μg)双蒸馏水，使得终浓度分别为100 μg/ml或200 μg/ml。
使用前10分钟加水。
使用前10分钟加水。
其他说明
仅用于生命科学研究。不可用于诊断。
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警示用语：
Warning-Warning
危险分类
Aquatic Chronic 3 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
does not flash
闪点(°C)
does not flash
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