产品名称
抗HA高亲和, from rat IgG1
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F10, monoclonal
form
lyophilized
packaging
pkg of 50 μg (11867423001)
pkg of 500 μg (11867431001)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
2-8°C
Quality Level
Biochem/physiol Actions
抗-HA高亲和识别9-氨基酸序列（YPYDVPDYA），这一序列来自人类流感血凝素（HA）蛋白。该表位同样识别融合蛋白，无论它的位置在哪里（N端，C端或中间）。
Analysis Note
Western印迹法功能测试。
Application
使用抗-HA高亲和用于检测天然流感血凝素蛋白和含有HA表位的重组蛋白，可用方法有：
- 斑点印迹法
- ELISA
- 免疫细胞化学
- 免疫沉淀
- Western印迹法
General description
抗-HA高亲和是一种HA-肽链（克隆3F10）的单克隆抗体。抗-HA高亲和识别HA肽链序列（YPYDVPDYA），这一序列来自人类流感血凝素蛋白。即使HA肽链表位通过表位标记，被引入不相关的重组蛋白，该抗体依然可以识别抗原决定簇。
Immunogen
98-106氨基酸来自人类流感病毒血凝素蛋白
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
工作浓度：偶联物工作浓度取决于用途和底物。
以下浓度可用作参考：
以下浓度可用作参考：
- ELISA：用于检测100ng/ml；用于涂层1至5μg/ml
- 免疫沉淀：0.5至5 μg/ml
- 蛋白质免疫印迹：50到200 ng/ml
加入0.5ml(11 867 423 001; 50 μg)或2.5ml(11 867 431 001; 500 μg)双蒸馏水，使得终浓度分别为100 μg/ml或200 μg/ml。
使用前10分钟加水。
使用前10分钟加水。
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signalword
Warning-Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
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