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检测方案
>98% (HPLC and SDS-PAGE)
形式
suspension
包装
pkg of 15 mL (05015952001 [settled resin volume])
pkg of 2 mL (11719416001 [settled resin volume])
pkg of 5 mL (11243233001 [settled resin volume])
制造商/商品名称
Roche
储存温度
2-8°C
一般描述
蛋白质G最初是从G148中分离出来，这是一种人组G链球菌菌株。这是一种细胞壁蛋白，有很高的IgG（免疫球蛋白G）亲和性。它公认的分子量是30000Da。它能和所有人IgG亚型互作，也能够结合小鼠、兔和山羊IgG。
特异性
蛋白质G是一种细胞壁蛋白质，从特殊细菌菌株中分离出来，对于来自不同物种的某些型免疫球蛋白有特异性结合位点。蛋白G几乎能够结合所有IgG亚型，但无法结合其它型免疫球蛋白。
结合能力：20mg人IgG（多克隆）/ml(IgG在pH7.0上样，pH2.8条件下采用20mg甘氨酸洗脱）
结构：重组蛋白G（E.coli，Mr=22000）共价偶联到4%交联琼脂糖珠：2.5mg蛋白质G（>98%纯化）/1ml胶。
结合能力：20mg人IgG（多克隆）/ml(IgG在pH7.0上样，pH2.8条件下采用20mg甘氨酸洗脱）
结构：重组蛋白G（E.coli，Mr=22000）共价偶联到4%交联琼脂糖珠：2.5mg蛋白质G（>98%纯化）/1ml胶。
应用
亲和层析使用蛋白G琼脂糖，就像蛋白A琼脂糖，是从许多物种中纯化IgG的方法。它可用作
- 泛素化实验
- 免疫沉淀实验的预清理剂
特点和优势
内容物
Preswollen胶，即用型，2ml或5ml胶（固定胶体积），溶于含14-19%乙醇（作为保鲜剂）的缓冲溶液中，未灭菌。
蛋白G内容物：2.5mg/ml preswollen胶
Preswollen胶，即用型，2ml或5ml胶（固定胶体积），溶于含14-19%乙醇（作为保鲜剂）的缓冲溶液中，未灭菌。
蛋白G内容物：2.5mg/ml preswollen胶
质量
纯度：>98% (HPLC, SDS-PAGE); 完全没有葡萄球菌肠毒素。
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Flam. Liq. 3
储存分类代码
3 - Flammable liquids
WGK
WGK 1
闪点(°F)
102.2 °F
闪点(°C)
39 °C
法规信息
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