检测方案
>98% (HPLC and SDS-PAGE)
形式
suspension
包装
pkg of 15 mL (05015979001 [settled resin volume])
pkg of 2 mL (11719408001 [settled resin volume])
pkg of 5 mL (11134515001 [settled resin volume])
制造商/商品名称
Roche
容量
>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)
储存温度
2-8°C
一般描述
重组蛋白A在蛋白A琼脂糖生产中的应用具有以下优点：它完全不含已知具有促有丝分裂作用的葡萄球菌肠毒素（分离自金黄色葡萄球菌的蛋白A中的污染物） 。固定化学可在广泛的pH和缓冲条件下提供高IgG的结合能力以及防漏稳定性。根据洗脱条件，凝胶可以使用30次以上。
蛋白A泄漏：<18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98％纯度，HPLC，SDS-Page/1ml凝胶
蛋白A，固定化。
蛋白A泄漏：<18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98％纯度，HPLC，SDS-Page/1ml凝胶
蛋白A，固定化。
特异性
蛋白A是从特定细菌菌株中分离的细胞壁蛋白，其具有来自不同物种的某些类免疫球蛋白的特异性结合位点。蛋白A可在不同程度结合IgM、IgA、IgD和大多数IgG亚类。
应用
从腹水或细胞培养上清液中纯化小鼠单克隆抗体。蛋白A或蛋白G琼脂糖亲和基质可用于从粗细胞提取物中纯化抗体 以及蛋白质的免疫沉淀或共免疫沉淀。它还被用于光活化-核糖核苷-增强的交联免疫沉淀（PAR-CLIP）分析。
组分
Preswollen凝胶，即用型，2ml或5ml（沉淀凝胶体积）悬浮于缓冲液中的凝胶，该缓冲液中含有20％乙醇作为保藏剂，非无菌。
蛋白A含量：3mg/ml预溶胀凝胶
蛋白A含量：3mg/ml预溶胀凝胶
质量
纯度：> 98％（HPLC，SDS-PAGE）；完全不含葡萄球菌肠毒素。
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Flam. Liq. 3
储存分类代码
3 - Flammable liquids
WGK
WGK 1
闪点(°F)
102.2 °F
闪点(°C)
39 °C
法规信息
监管及禁止进口产品
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