产品名称
丙酮酸激酶(PK), from rabbit muscle
biological source
rabbit muscle
form
suspension
specific activity
~200 units/mg protein (at 25 °C (500 U/mg at 37 °C) with PEP as the substrate.)
packaging
pkg of 1 mL (10128155001 [10 mg])
pkg of 10 mL (10128163001 [100 mg])
manufacturer/tradename
Roche
optimum pH
7.0-7.5
storage temp.
2-8°C
Quality Level
Analysis Note
污染物：<0.001%的GK，分别<0.002%的“ NADH氧化酶”和ATP酶，分别<0.01%的烯醇酶、LDH和肌激酶
Application
丙酮酸激酶已被用于测量ATP酶活性和腺苷酸浓度。
Biochem/physiol Actions
通过利用质子，丙酮酸激酶可催化磷酸烯醇丙酮酸和ADP不可逆转化为丙酮酸和ATP。第一步是将磷酸基团从磷酸烯醇丙酮酸转移至ADP，并形成丙酮酸的结合型烯醇化物和ATP。在第二步中，将质子添加到烯醇化物，生成酮形式的丙酮酸。此外，该酶还具有其他活性，如ATP和重碳酸盐依赖性ATP酶、氟化物和羟胺的磷酸化、乙醇酸盐的ATP依赖性磷酸化以及草酰乙酸的脱羧反应。
General description
ATP:丙酮酸2-O-磷酸转移酶
丙酮酸激酶的摩尔质量为237,000，以四聚体形式存在。该四聚体的每个多肽链的摩尔质量为57,200。该酶包含两个相同的催化颗粒，称为启动子。这些启动子各自含有两条多肽链。每个启动子都含有一个Mn2+位点和一个磷酸烯醇丙酮酸位点。
丙酮酸激酶的摩尔质量为237,000，以四聚体形式存在。该四聚体的每个多肽链的摩尔质量为57,200。该酶包含两个相同的催化颗粒，称为启动子。这些启动子各自含有两条多肽链。每个启动子都含有一个Mn2+位点和一个磷酸烯醇丙酮酸位点。
Other Notes
仅用于生命科学研究。不可用于诊断。
Physical form
悬浮于3.2 M硫酸铵溶液中，pH约为6
Preparation Note
活化剂：PK需要Mg2+（或Mn2+，Co2+）以及 K+(或NH4+，Rb+)以发挥全部活性。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
法规信息
动植物源性产品
低风险生物材料
此项目有
Ilana M Nodelman et al.
EMBO reports, 14(12), 1098-1103 (2013-10-16)
Chromatin remodellers are ATP-dependent motor proteins that physically reposition and reorganize nucleosomes. Chd1 and Iswi-type remodellers possess a DNA-binding domain (DBD) needed for efficient nucleosome mobilization; however, it has not been clear how this domain physically contributes to remodelling. Here
Monika Ostaszewska et al.
Journal of plant physiology, 171(7), 549-558 (2014-03-25)
Sulphur, as a constituent of amino acids (cysteine and methionine), iron-sulphur clusters, proteins, membrane sulpholipids, glutathione, glucosinolates, coenzymes, and auxin precursors, is essential for plant growth and development. Absence or low sulphur concentration in the soil results in severe growth
Metabolic control and structure of glycolytic enzymes. 3. Dissociation and subunit structure of rabbit muscle pyruvate kinase.
M A Steinmetz et al.
Biochemistry, 5(4), 1399-1405 (1966-04-01)
T M Larsen et al.
Biochemistry, 33(20), 6301-6309 (1994-05-24)
The molecular structure of rabbit muscle pyruvate kinase, crystallized as a complex with Mn2+, K+, and pyruvate, has been solved to 2.9-A resolution. Crystals employed in the investigation belonged to the space group P1 and had unit cell dimensions a
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持