产品名称
甲酸脱氢酶, from Candida boidinii
biological source
yeast (Candida boidini)
recombinant
expressed in E. coli
form
lyophilized
specific activity
~0.4 units/mg protein (At 25 °C with formate as the substrate.)
mol wt
74 kDa
packaging
pkg of 250 U (10837016001)
pkg of 80 U (10244678001)
manufacturer/tradename
Roche
concentration
≥10-20 % (w/w)
technique(s)
activity assay: suitable
color
off-white
optimum pH
7.5-8.5
solubility
water: soluble 10 g/L
suitability
suitable for gel electrophoresis
UniProt accession no.
application(s)
life science and biopharma
foreign activity
ADH <0.0500%
LDH <0.0500%
MDH <0.1000%
storage temp.
2-8°C
Quality Level
Analysis Note
污染物：<0.005％的LDH和ADH，<0.1％的MDH
Application
最常用于NADH的辅因子再循环系统。
Biochem/physiol Actions
底物特异性和Km：
甲酸脱氢酶仅与甲酸盐（在30℃和pH 7.5条件下，Km = 13 mM；在25℃和pH 7.5条件下，11mM）和NAD（在30℃和pH 7.5条件下，Km 0.09 mM）反应。它不与乙酸盐、草酸盐、乳酸盐、琥珀酸盐、丙酸盐或抗坏血酸盐反应，也不会还原NADP。
甲酸脱氢酶仅与甲酸盐（在30℃和pH 7.5条件下，Km = 13 mM；在25℃和pH 7.5条件下，11mM）和NAD（在30℃和pH 7.5条件下，Km 0.09 mM）反应。它不与乙酸盐、草酸盐、乳酸盐、琥珀酸盐、丙酸盐或抗坏血酸盐反应，也不会还原NADP。
General description
甲酸盐:NAD + 氧化还原酶
甲酸脱氢酶是左旋体特异性2-羟基酸脱氢酶超家族的成员。它是由两个相同亚基组成的二聚体。该酶具有一个半胱氨酸残基，它对于酶的活性或结构完整性至关重要。
甲酸脱氢酶是左旋体特异性2-羟基酸脱氢酶超家族的成员。它是由两个相同亚基组成的二聚体。该酶具有一个半胱氨酸残基，它对于酶的活性或结构完整性至关重要。
Other Notes
一单位(U)甲酸脱氢酶在+25 °C、pH 7.6下将1 μmol甲酸氧化成CO2。上述测定中，每氧化1 mmol甲酸将产生1mmol NADH。
仅用于生命科学研究。不可用于诊断。
Preparation Note
干燥储存
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
Katja Schirwitz et al.
Protein science : a publication of the Protein Society, 16(6), 1146-1156 (2007-05-26)
The understanding of the mechanism of enzymatic recovery of NADH is of biological and of considerable biotechnological interest, since the essential, but expensive, cofactor NADH is exhausted in asymmetric hydrogenation processes, but can be recovered by NAD(+)-dependent formate dehydrogenase (FDH).
Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
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