重组
expressed in E. coli (AP401 (k))
形式
solution
用途
sufficient for 100 reactions
sufficient for 20 reactions
包装
pkg of 1,000 U (11785826001 [20 μl])
pkg of 5,000 U (11785834001 [100 μl])
制造商/商品名称
Roche
参数
42-43 °C optimum reaction temp.
技术
RT-PCR: suitable
RT-qPCR: suitable
输入
purified RNA
检测方法
probe-based
储存温度
−20°C
一般描述
Expand™逆转录酶是一种RNA指导的DNA聚合酶，经过优化在两步法 RT-PCR检测中可以将cDNA扩增为13.5 kb 片段。该酶是莫洛尼鼠白血病病毒逆转录酶（M-MuLV-RT）的基因工程产品。核糖核酸酶H序列中的A点突变会使核糖核酸酶H活性降低至无法检出的水平。与采用天然M-MuLV-RT相比可以得到明显的改进，包括全长cDNA转录本数量更多且转录本更长
特异性
热灭活：95 °C下处理2分钟
适用于3kb RT-PCR反应的逆转录酶
适用于3kb RT-PCR反应的逆转录酶
应用
Expand™逆转录酶已被用于实时PCR 分析，可以使分离的RNA转化，进入cDNA。它还被用于逆转录（RT）。Expand™逆转录酶也可用于分析 cDNA 的序列并与基因组序列进行比较。 可以获得有关间隔顺序（内含子）和基因组重组的重要信息。
特点和优势
- 获得更多的全长cDNA转录本和更长的高达13.5kb的转录本
- 没有核糖核酸酶 H活性
- 高效地在二级结构中转录总RNA、mRNA、病毒RNA和富含RNA物质。
- 两步法 RT-PCR检测的理想选择
- 在PCR反应前不需要纯化cDNA
包装
包含3个组分。
单位定义
一单位逆转录酶的定义为，在+37 °C下，以 poly(A)+ × (dT)15为底物，将10 nmol TMP整合为酸性不可溶产物所需的酶量。
体积活性：50 U/μl
体积活性：50 U/μl
其他说明
仅用于生命科学研究。不可用于诊断。
法律信息
Expand is a trademark of Roche
仅试剂盒组分
产品编号
说明
- Expand Reverse Transcriptase 50 U/μl
- Expand Reverse Transcriptase Buffer 5x concentrated
- DTT Stock Solution 100 mM
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°C)
does not flash
法规信息
常规特殊物品
Edwin Yunhao Gong et al.
Methods in molecular biology (Clifton, N.J.), 1030, 195-203 (2013-07-04)
We have developed a duplex real-time RT-PCR assay for profiling antiviral inhibitors of four dengue virus (DENV) serotypes. In this assay, the primers and the probe for amplifying DENV were designed in the conserved regions of the genome after aligned
M Hatzfeld et al.
The Journal of cell biology, 149(1), 209-222 (2000-04-04)
Plakophilin 1, a member of the armadillo multigene family, is a protein with dual localization in the nucleus and in desmosomes. To elucidate its role in desmosome assembly and regulation, we have analyzed its localization and binding partners in vivo.
Kathrin Ikels et al.
PloS one, 9(8), e105349-e105349 (2014-08-22)
The metabolic syndrome is a worldwide problem mainly caused by obesity. FTO was found to be a obesity-risk gene in humans and FTO deficiency in mice led to reduction in adipose tissue. Thus, FTO is an important factor for the
André Spychala et al.
PloS one, 14(2), e0211937-e0211937 (2019-02-08)
Initially, the function of the fat mass and obesity associated (Fto) gene seemed to be primarily the regulation of the body weight. Here we show that loss of Fto results in a hyperactivation of the hypothalamic-pituitary-adrenal (HPA) axis. In consequence
Christoph Gerhardt et al.
PloS one, 8(2), e57545-e57545 (2013-03-08)
Ventricular septal defects (VSDs) are the most common congenital heart defects in humans. Despite several studies of the molecular mechanisms involved in ventricular septum (VS) development, very little is known about VS-forming signaling. We observed perimembranous and muscular VSDs in
相关内容
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
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