产品名称
蛋白内切酶Lys-C 测序级, from Lysobacter enzymogenes
biological source
bacterial (Lysobacter enzymogenes)
form
lyophilized
specific activity
≥200 units/mg protein (at 25 °C, with Chromozym PL)
mol wt
33 kDa by SDS-PAGE (reducing)
purified by
electrophoresis
packaging
pkg of 3 × 5 μg (11047825001)
pkg of 5 μg (11420429001)
manufacturer/tradename
Roche
storage condition
(Keep container tightly closed in a dry and well-ventilated place.)
technique(s)
activity assay: suitable
color
white
optimum pH
8.5-8.8
pH range
5-12
solubility
water: soluble
suitability
suitable for enzyme test
UniProt accession no.
storage temp.
2-8°C
Application
使用内蛋白酶赖氨酸-碳测序等级进行蛋白质结构分析和序列分析。它适用于消化溶液、聚丙烯酰胺凝胶或吸水膜中的蛋白质。
Biochem/physiol Actions
这种酶是一种丝氨酸蛋白酶，能特异性水解赖氨酸羧基侧的酰胺、酯和肽键（在三-盐酸缓冲液中，pH 7.0至9.0）。
热失活：酶可以通过加热（100℃下5分钟）或TCA变性。
热失活：酶可以通过加热（100℃下5分钟）或TCA变性。
General description
内蛋白酶赖氨酸-碳测序等级是从产酶溶血杆菌中分离出来的，是一种高度纯化和特异的蛋白酶。来自产酶溶杆菌的内切蛋白酶 Lys-C是肽酶S1家族的一员。它主要用于蛋白质序列分析中多肽链的初始断裂。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
储存条件（工作溶液）：+2至+8℃
冻干内蛋白酶赖氨酸-碳测序等级在50 & # 956；L双蒸水中重构。生成缓冲液浓度为50 mM Hepes、pH 8.0、10 mM乙二胺四乙酸和5 mg/ml棉子糖。为避免自溶，培养温度不应超过37℃。
在4 M尿素中稳定。
在4 M尿素中稳定。
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
H Alexander Ebhardt et al.
Rapid communications in mass spectrometry : RCM, 28(24), 2735-2743 (2014-11-08)
Tandem mass (MS/MS) spectra generated by collision-induced dissociation (CID) typically lack redundant peptide sequence information in the form of e.g. b- and y-ion series due to frequent use of sequence-specific endopeptidases cleaving C- or N-terminal to Arg or Lys residues.
Heterologous expression and pro-peptide supported refolding of the high specific endopeptidase Lys-C
Stressler T, et al.
Protein Expression and Purification, 118, 31-38 (2016)
Tao Xu et al.
Nature protocols, 4(3), 325-332 (2009-02-21)
This protocol outlines the essential steps of mass spectrometry-based analysis of protein samples that can be used to identify post-translational arginylation. We describe special considerations for sample preparation and digestion, mass spectrometry analysis using high-precision instruments, database searching for the
Jung Hun Oh et al.
Journal of proteome research, 10(3), 1406-1415 (2011-01-14)
Many efforts have been made to discover novel bio-markers for early disease detection in oncology. However, the lack of efficient computational strategies impedes the discovery of disease-specific biomarkers for better understanding and management of treatment outcomes. In this study, we
Jason A Wall et al.
American journal of physiology. Heart and circulatory physiology, 291(5), H2462-H2472 (2006-06-13)
Ischemia-reperfusion (I/R) has critical consequences in the heart. Recent studies on the functions of I/R-activated kinases, such as p38 mitogen-activated protein kinase (MAPK), showed that I/R injury is reduced in the hearts of transgenic mice that overexpress the p38 MAPK
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