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DIGUTP-RO

Roche

地高辛-11-UTP

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UNSPSC代码:
12352200
NACRES:
NA.21

检测方案

≥85% (HPLC)

质量水平

100

形式

solution

分子量

(Mr = 1106.7 (DIG-11-UTP-Li4))

包装

pkg of 25 μL (11209256910 [10 mM])
pkg of 57 μL (03359247910 [3.5 mM])

制造商/商品名称

Roche

储存温度

−20°C

相关类别

一般描述

地高辛(DIG)-11-尿苷三磷酸(UTP)以四锂盐溶液形式提供，浓度为3.5mM(200nmol)或10mM(250nmol)。它被用作是SP6、T3和T7 RNA聚合酶的底物。它可以在体外转录反应中取代UTP，从而以35:65％的比例进行RNA的DIG标记。

应用

地高辛-11-UTP已用于标记以下应用中的RNA探针：
  • 原位杂交
  • 原位逆转录酶(RT)-聚合酶链式反应(PCR)
  • 荧光原位杂交(FISH)

生化/生理作用

利用ATP、GTP、CTP、UTP和地高辛-11-UTP和相应的RNA聚合酶可分别对具有T7、SP6或T3启动子的线性模板DNA进行 体外 转录。随后，可以用抗地高辛-AP（碱性磷酸酶）Fab片段抗体、地高辛核酸检测试剂盒或地高辛核酸发光检测试剂盒检测标记的RNA。

质量

典型分析：85% DIG-11-UTP (HPLC，峰面积%)
使用DIG RNA标记试剂盒进行功能测试。

化学式：C43H61N4O22P3Li4

其他说明

仅用于生命科学研究。不可用于诊断。

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H302

危险分类

Acute Tox. 4 Oral

储存分类代码

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash

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