产品名称
肌酸激酶（CK）, from rabbit muscle
biological source
rabbit muscle
form
lyophilized
packaging
pkg of 100 mg (10127566001)
pkg of 500 mg (10736988001)
manufacturer/tradename
Roche
color
white to light yellow
optimum pH
6.0-7.0(reverse reaction)
9.0(forward reaction)
solubility
water: soluble
suitability
suitable for immunoprecipitation
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
sample preparation
foreign activity
ATPase <0.001%
HK <0.001%
myokinase <0.001%
storage temp.
2-8°C
Quality Level
Gene Information
rabbit ... CKM(100009056)
Analysis Note
污染物：＆lt;0.001％ ATP酶，HK和肌激酶，每种
Application
肌酸激酶已被用作体外翻译测定中使用的翻译缓冲液的组分，并且作为能量再生混合物的组分。
Biochem/physiol Actions
肌酸激酶（CK）是一种胍基激酶，消耗ATP催化肌酸可逆磷酸化为磷酸肌酸。它在具有高能量需求的细胞中快速再生ATP中起着至关重要的作用。人血液中CK水平的增加表明神经系统和心肌疾病、恶性低温和某些肿瘤。金属（例如Mg、Mn和Ca）充当CK的辅助因子。它还形成蛋白激酶C的底物，蛋白激酶C是参与细胞生长的重要信号分子。人们发现 CK的酶促反应产物，磷酸肌酸，可以阻止 HIV（人类免疫缺陷病毒）在巨噬细胞中复制。
Features and Benefits
具体活动：在+ 25°C下大约 350U/mg冻干物（+ 37°C下约800U/mg 冻干物），其中磷酸肌酸和ADP作为底物，并且N-乙酰基-L-半胱氨酸作为活化剂。
General description
25℃（37℃，800 U/mg冻干物），以磷酸肌酸和ADP为底物，N-乙酰-L-半胱氨酸为激活剂。
Other Notes
一个单位CK活性（+ 25°C）= 2.3 U（+ 37°C），肌酸蛋白-P和ADP 作为底物，NAcCys作为重活化剂。
仅用于生命科学研究。不可用于诊断。
Physical form
冻干粉，稳定
Preparation Note
储存条件（工作液）：常规应用中的工作溶液和储存条件：
溶于0.1M 咪唑缓冲液中；等分并储存在- 15至-25°C。
在-15至-25°C稳定约四周。
避免反复冻融。
溶于0.5％ NaHCO3溶液中。
不要冷冻。避光储存。
在2至8°C条件下储存时，稳定2-3天。
或
用含有0.2mM DTT的30mM甘氨酸缓冲液（pH9.0）溶解。
在2至8°C条件下储存时，稳定2-3天。
溶于0.1M 咪唑缓冲液中；等分并储存在- 15至-25°C。
在-15至-25°C稳定约四周。
避免反复冻融。
溶于0.5％ NaHCO3溶液中。
不要冷冻。避光储存。
在2至8°C条件下储存时，稳定2-3天。
或
用含有0.2mM DTT的30mM甘氨酸缓冲液（pH9.0）溶解。
在2至8°C条件下储存时，稳定2-3天。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
动植物源性产品
低风险生物材料
此项目有
Irina M Kuznetsova et al.
Biochimica et biophysica acta, 1596(1), 138-155 (2002-05-02)
GdmCl-induced unfolding of rabbit muscle creatine kinase, CK, has been studied by a variety of physico-chemical methods including near and far UV CD, SEC, intrinsic fluorescence (intensity, anisotropy and lifetime) as well as intensity and lifetime of bound ANS fluorescence.
J K Rao et al.
FEBS letters, 439(1-2), 133-137 (1998-12-16)
The crystal structure of rabbit muscle creatine kinase, solved at 2.35 A resolution by X-ray diffraction methods, clearly identified the active site with bound sulfates surrounded by a constellation of arginine residues. The putative binding site of creatine, which is
Aurélie M Rakotondrafara et al.
Nature protocols, 6(5), 563-571 (2011-04-30)
Much of the regulation of gene expression occurs at the level of protein synthesis. In addition to the canonical translation factors, a multitude of proteins and microRNAs (miRNAs) act as regulatory trans-acting factors. Mechanistic analysis of translational control benefits from
Marta Teperek et al.
International journal of molecular sciences, 15(9), 16719-16740 (2014-09-23)
Spermatozoa are more efficient at supporting normal embryonic development than spermatids, their immature, immediate precursors. This suggests that the sperm acquires the ability to support embryonic development during spermiogenesis (spermatid to sperm maturation). Here, using Xenopus laevis as a model
Marta Teperek et al.
Genome research, 26(8), 1034-1046 (2016-04-02)
For a long time, it has been assumed that the only role of sperm at fertilization is to introduce the male genome into the egg. Recently, ideas have emerged that the epigenetic state of the sperm nucleus could influence transcription
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持