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生物来源
Pseudomonas fluorescens
质量水平
重组
expressed in E. coli
表单
suspension
比活
~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.)
分子量
64,000 Da
包装
pkg of 1 mL (10104973001 [1 mg])
pkg of 1 mL (10104981001 [5 mg])
制造商/商品名称
Roche
技术
activity assay: suitable
颜色
white
最佳pH
8.4 (0.1 M in Tris buffer)
pH值范围
4.6-9.2
溶解性
water: soluble
适用性
suitable for enzyme test
UniProt登记号
异质活性
ADH <0.01%
LDH <0.5%
NADH oxidase <0.1%
galactosidase <0.01%
储存温度
2-8°C
一般描述
β-Galactose Dehydrogenase plays a crucial role in galactose metabolism by catalyzing the pyridine-nucleotide-dependent dehydrogenation of β-D-galactopyranose to yield NADH and D-galactono-l,5-1actone. D-galactose dehydrogenase from P. fluorescens is a member of the oxidoreductases family. It is composed of two identical subunits and has a molecular weight of 64,000. Additionally, the enzyme contains two binding sites.
特异性
Galactose dehydrogenase will oxidize D-galactose [Km = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32).
应用
β-Galactose Dehydrogenase has been used as a reporter enzyme in neuraminidase (NA) kinetic parameters for the natural substrates 6′-Sialyl-N-acetyllactosamine (6SLN) and 3′-Sialyl-N-acetyllactosamine (3SLN).
质量
Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH
序列
Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D).
单位定义
One unit galactose dehydrogenase will oxidize 1 mol of β-D-galactose in one minute at +25 °C and pH 8.6.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.
外形
Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6
其他说明
仅用于生命科学研究。不可用于诊断。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
No data available
闪点(°C)
No data available
法规信息
常规特殊物品
Biophysical Measurement of the Balance of Influenza A Hemagglutinin and Neuraminidase Activities*
Benton, et al.
The Journal of Biological Chemistry, 290, 6516-6521 (2015)
[29] d-Galactose dehydrogenase from Pseudomonas fluorescens
E. Maier and G. Kurz
Methods in Enzymology, 89.0, 176-181 (1982)
Modelling severe Staphylococcus aureus sepsis in conscious pigs: are implications for animal welfare justified?
Olsen, et al.
BMC Research Notes, 9 (2016)
Recombinant D-galactose dehydrogenase partitioning in aqueous two-phase systems: effect of pH and concentration of PEG and ammonium sulfate
Kianmehr A, et al.
Bioresources and bioprocessing, 1.0 (2014)
Biophysical measurement of the balance of influenza a hemagglutinin and neuraminidase activities
Benton D, et al.
The Journal of biological chemistry, 290(10), 6516-6521 (2015)
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