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安全信息

XTGHP-RO

Roche

X-tremeGENE™ HP DNA转染试剂

High-performance polymer reagent for transfecting many cell lines

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25 G
CN¥852.42
100 G
CN¥2,903.41
250 G
CN¥6,381.78
1 KG
CN¥17,876.60

CN¥852.42

国内现货，预计发货时间April 14, 2025详情

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25 G
CN¥852.42
100 G
CN¥2,903.41
250 G
CN¥6,381.78
1 KG
CN¥17,876.60

About This Item

UNSPSC代码:
41106502
NACRES:
NA.55

CN¥852.42

国内现货，预计发货时间April 14, 2025详情

等级

Molecular Biology

质量水平

100

表单

liquid (aqueous solution)

用途

 mL (suitable for 165 transfections)

包装

pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)

制造商/商品名称

Roche

技术

transfection: suitable

储存温度

−20°C

相关类别

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Gene Information

Gene Information

Gene Information

Gene Information

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

一般描述

X-tremeGENE HP DNA转染试剂是一款多组分试剂，能够与DNA形成复合物，并将其递送至动物或昆虫细胞中。

特点和优势

X-tremeGENE HP DNA转染试剂是一种高效转染试剂，可成功转染多种真核细胞，包括昆虫细胞和难转染细胞系（如Sf9、U-2 OS、MEF、MCF7、Hep G2、PC-3、HeLa、HT-29、HT-1080、K-562、RAW 264.7、Jurkat、PC-12和HCT 116）。

  • 简单易用的非脂质体试剂 无动物源性成分，室温稳定，经0.2 μm滤膜过滤，并且在有血清培养液中仍保持活性。
  • 高转染效率 对于原代细胞和使用其它试剂难转染的肿瘤细胞系有高转染效率。
  • 结果生理相关性好 使用细胞毒性低的试剂。
  • 满足高通量实验 实现简单一致的靶标评估。

质量

每一批次的X-tremeGENE HP DNA转染试剂都经过严格的质量控制流程测试，确保性能合规的标准性。在质量测试环节，首先使用X-tremeGENE HP DNA转染试剂（试剂DNA比为3:1μl/μg DNA）向细胞转染一个报告基因载体。再通过化学荧光检测法监测报告基因活性。最后使用一条标准曲线对每孔的重组蛋白总量进行定量，以确定产品达到规格。

外形

本产品溶于80%乙醇中，经0.2 μm滤膜过滤。无人源或动物源成分。次数：使用标准步骤时，在96孔板内，1ml X-tremeGENE™ HP DNA转染试剂可以进行10,000次转染。

其他说明

仅用于生命科学研究。不可用于诊断。

法律信息

X-tremeGENE is a trademark of Roche

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象形图

FlameExclamation mark
GHS02,GHS07

警示用语：

Danger

危险声明

H225 - H319

危险分类

Eye Irrit. 2 - Flam. Liq. 2

储存分类代码

3 - Flammable liquids

WGK

WGK 1

闪点(°F)

334.4 °F

闪点(°C)

168 °C

法规信息

危险化学品
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    47244400
    32223900
    24223000
    19908900
    14528400

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    Richard R W Brady et al.
    Carcinogenesis, 32(7), 1069-1077 (2011-05-10)
    Long-term aspirin or related non-steroidal anti-inflammatory drugs (NSAIDs) ingestion can protect against colorectal cancer (CRC). NSAIDs have a pro-apoptotic activity and we have shown that stimulation of the nuclear factor-kappaB (NF-κB) pathway is a key component of this pro-apoptotic effect.
    Ido D Weiss et al.
    Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 14(1), 106-114 (2011-02-25)
    Expression of CXCR4 in cancers has been correlated with poor prognosis and increased metastasis. Quantifying CXCR4 expression non-invasively might aid in prognostication and monitoring therapy. We evaluated a radiolabeled antagonist of CXCR4, ⁶⁴Cu-AMD3100, as a positron-emitting imaging agent. CXCR4-transfected or
    Takashi Yoshino et al.
    Cellular and molecular neurobiology, 31(7), 1009-1020 (2011-04-27)
    A synthetic analog of sphingosine named FTY720 (Fingolimod), phosphorylated by sphingosine kinase-2, interacts with sphingosine-1-phosphate (S1P) receptors expressed on various cells. FTY720 suppresses the disease activity of multiple sclerosis (MS) chiefly by inhibiting S1P-dependent egress of autoreactive T lymphocytes from
    Laura M Griffin et al.
    Methods in molecular biology (Clifton, N.J.), 1195, 219-238 (2013-11-28)
    Human papillomaviruses (HPVs) are non-enveloped DNA viruses that are highly tropic for mucosal and cutaneous epithelia. The HPV life cycle is tightly linked to epithelial cell differentiation, where HPVs only infect the basal proliferating keratinocytes, and progeny virus assembly and
    Abazar Roustazadeh et al.
    SpringerPlus, 5(1), 711-711 (2016-07-05)
    Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799
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    Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.

    实验方案

    使用X-tremeGENE HP转染试剂进行慢病毒生产

    慢病毒是一种用于在研究应用中转染多种细胞类型的强大工具。

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    X-tremeGENE™ HP DNA转染试剂实验方案

    约转染前24小时接种转染细胞，确保细胞处于最佳密度（7090 %融合度）。

    X-tremeGENE™ HP DNA Transfection Reagent Protocol

    Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

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