质量水平
形式
suspension
比活
~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
包装
vial of 2 mL (10127833001)
vial of 5 mL (10127841001)
制造商/商品名称
Roche
浓度
5 mg/mL
杂质
<0.1% LDH
<5% MDH
最佳pH
6.2-6.9(for reduction)
7-9(for oxidation)
运输
wet ice
储存温度
2-8°C
一般描述
3-羟基丁酸脱氢酶 (3-HBDH),从 球形红杆菌中获得 ,通常用于酮体的定量,如 D-3-羟基丁酸和乙酰乙酸。
应用
3-羟基丁酸脱氢酶 (3-HBDH) 选择性氧化 (R)-3-羟基单羧酸,或逆反应。
生化/生理作用
3-羟基丁酸脱氢酶 (3-HBDH) 是一种线粒体酶,以 NAD 为辅酶,催化 3-羟基丁酸 (3 HB) 可逆氧化为乙酰乙酸。在哺乳动物中,乙酰辅酶 A 在两种途径之一被代谢,生成乙酰乙酸和 D-3-羟基丁酸,与丙酮一起被称为酮体。3-HBDH 可逆性地将该游离乙酰乙酸还原为 D-3-羟基丁酸。在糖尿病酮症酸中毒 (DKA) 患者中,3 HB 和乙酰乙酸的比例可高达 10:1,而正常比例为 1:1。3-HBDH 酶可用于检测尿、血清、血标本中 3 HB 的量。
质量
污染物: 0.1%LDH,5%MDH
外形
悬浮于3.2 M硫酸铵溶液中,pH约为6
制备说明
稳定剂: NADH,Ca2 +
其他说明
仅用于生命科学研究。不可用于诊断。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
No data available
闪点(°C)
No data available
法规信息
常规特殊物品
Acta crystallographica. Section F, Structural biology and crystallization communications, 61(Pt 1), 36-38 (2006-03-02)
A recombinant form of D-3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) from Pseudomonas fragi has been crystallized by the hanging-drop method using PEG 3000 as a precipitating agent. The crystals belong to the orthorhombic group P2(1)2(1)2, with unit-cell parameters a = 64.3, b
The Biochemical journal, 241(1), 297-300 (1987-01-01)
The enzymic determination of D-3-hydroxybutyrate and acetoacetate normally involves the use of 3-hydroxybutyrate dehydrogenase (HBDH, EC 1.1.1.30) of bacterial origin. We show that HBDH from Rhodopseudomonas spheroides (BCL, grade II) contains a 3-hydroxyisobutyrate dehydrogenase (HIBDH) activity: activity with 3-hydroxyisobutyrate as
IET nanobiotechnology, 7(1), 1-6 (2013-05-28)
Precise detection of 3-hydroxybutyrate (HB) in biological samples is of great importance for management of diabetic patients. In this study, an HB biosensor based on single-walled carbon nanotubes (SWCNTs)-modified screen-printed electrode (SPE) was developed to determine the concentration of HB
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