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质量水平
用途
sufficient for 500 reactions
保质期
≤18 mo.
特点
Fast PCR
Multiplex PCR
dNTPs included
hotstart: no
包装
pkg of 100 U (KK5008)
pkg of 250 U (KK5009)
制造商/商品名称
Roche
技术
PCR: suitable
输入
purified DNA
储存温度
−20°C
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一般描述
KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
应用
KAPA2G Fast PCR Kit has been used in:
- PCR with confronting 2 -pair primers assay (PCR-CTPP)
- Routine PCR
- Fast PCR
- Genotyping
生化/生理作用
KAPA2G Fast DNA Polymerase generated DNA fragments is useful in sequencing restriction enzyme digestion and cloning experiments. KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities like wild-type Taq, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.
特点和优势
Reduce cycling times up to 75% :
High speed, higher performance :
Quick Notes :
- Extension times as low as 1 sec per kilobase
- Broad coverage of both AT- and GC-rich targets
High speed, higher performance :
- Single copy sensitivity from complex targets
- Increased yields
Quick Notes :
- KAPA2G Fast PCR Kits with dNTPs contain the engineered KAPA2G Fast DNA Polymerase, developed for fast PCR.
- Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
- No need for specialized instrumentation or PCR consumables.
- Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
- Use 0.5 U KAPA2G Fast DNA Polymerase per 25 μL reaction, or less for smaller volumes.
- For high reaction efficiency, do not exceed 25 μL reaction volumes.
- KAPA2G Buffer A contains 1.5 mM MgCl2 at 1X.
- Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
质量
Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast Ready Mix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
制备说明
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.
其他说明
For Research Use Only. Not for use in diagnostic procedures.
仅试剂盒组分
产品编号
说明
- KAPA2G Fast Standard or HotStart® DNA Polymerase (5 U/μL)
- 5X KAPA2G Buffer A with MgCl2
- MgCl2 25 mM
警示用语：
Warning
危险声明
危险分类
Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
Nevin M Al Azhary et al.
Asian Pacific journal of cancer prevention : APJCP, 17(11), 4945-4949 (2016-12-30)
Background: Breast cancer is the commonest cancer in Egyptian females. Nrf2 is involved in oxidative stress while P73 functions in response to DNA damage. This study aimed to assess the role of Nrf2 promoter and P73 G4C14 to A4T14 SNPs
Klotho G395A Polymorphism in Egyptian Patients with Diabetic Nephropathy and Hemodialysis
Nasr HG Abd El-Aziz, et al.,
Al-Azhar Medical Journal, 331, 1-13 (2013)
Kazuyuki Hoshijima et al.
Developmental cell, 51(5), 645-657 (2019-11-12)
Inconsistent activity limits the use of CRISPR-Cas9 in zebrafish. We show supernumerary guanine nucleotides at the 5' ends of single guide RNAs (sgRNAs) account for diminished CRISPR-Cas9 activity in zebrafish embryos. Genomic sequences can be targeted consistently with extremely high
Elisa Bergamo et al.
Journal of virological methods, 181(1), 125-130 (2012-01-24)
The potential advantage of using fast PCR to detect human herpesvirus 8 (HHV-8) was tested by running a rapid cycling protocol (5s-steps) in one standard and two fast ramping thermal cyclers to evaluate the performance of 8 different fast reagents.
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An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
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