biological source
rat
conjugate
fluorescein conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F10, monoclonal
form
lyophilized
packaging
pkg of 25 μg
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
−20°C
General description
高亲和力抗 HA 荧光素是一种单克隆抗体,适用于对荧光素偶联的HA标记重组蛋白Fab片段的高灵敏度检测。
高亲和力抗 HA 荧光素可识别来源于人血凝素的HA肽序列(YPYDVPDYA)。即使通过称为“表位标记”的技术将HA肽表位引入不相关的重组蛋白中,抗体也可识别其抗原决定簇。
内容
冻干品,稳定
高亲和力抗 HA 荧光素可识别来源于人血凝素的HA肽序列(YPYDVPDYA)。即使通过称为“表位标记”的技术将HA肽表位引入不相关的重组蛋白中,抗体也可识别其抗原决定簇。
内容
冻干品,稳定
Immunogen
来自人流感病毒血凝素蛋白的氨基酸98至106
Application
高亲和力抗 HA 荧光素已被用于中国仓鼠卵巢活细胞的免疫荧光标记 和神经元细胞培养物的免疫细胞化学分析。
Biochem/physiol Actions
高亲和力抗 HA 荧光素 (3F10) 可识别来源于人流感血凝素 (HA) 蛋白的9 个氨基酸序列 YPYDVPDYA。无论其位置如何(N 端、C 端或内部),该表位也可在融合蛋白中识别。
Preparation Note
稳定剂:蛋白质稳定剂
工作浓度:流式细胞术:1-5 μg/ml
免疫荧光:1-5 μg/ml
偶联物的工作浓度取决于具体应用和底物。应根据指南确定浓度。
储存条件(工作溶液):重悬抗体可以在2至8°C温度下稳定保存两个月。 分装品可以在-15至-25°C下保存长达六个月。
注意:避免反复冻融。
工作浓度:流式细胞术:1-5 μg/ml
免疫荧光:1-5 μg/ml
偶联物的工作浓度取决于具体应用和底物。应根据指南确定浓度。
储存条件(工作溶液):重悬抗体可以在2至8°C温度下稳定保存两个月。 分装品可以在-15至-25°C下保存长达六个月。
注意:避免反复冻融。
加入 0.5 ml 双蒸水至最终浓度 50μg/ml。使用前再水合 10 分钟。
Analysis Note
高亲和力抗 HA 荧光素抗体是通过HA标记融合蛋白的流式细胞术分析进行功能检测的
Other Notes
仅用于生命科学研究。不可用于诊断。
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
低风险生物材料
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