生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
monoclonal
形式
lyophilized
包装
pkg of 100 μg
制造商/商品名称
Roche
同位素/亚型
IgG1
储存温度
2-8°C
一般描述
Anti-His 6 是针对 His 6 标签蛋白的单克隆抗体。抗 His 6 抗体特异性识别天然蛋白和重组蛋白的 6 个连续组氨酸残基的抗原表位。抗体与天然和变性组氨酸标记的融合蛋白反应，与蛋白序列中组氨酸标记的位置无关。
特异性
抗 His 6 抗体特异性识别天然和重组蛋白中 6 个连续组氨酸残基 (His 6 ) 的抗原表位。它与天然的和变性的组氨酸标记的融合蛋白反应，与蛋白序列中组氨酸标记的位置无关。此外，抗 His 6 抗体优先识别具有高灵敏度的 C 末端 His 6 表位。无论使用何种表达系统，Anti-His 6 均可特异性且灵敏地检测组氨酸标记蛋白。
该抗体识别天然和重组蛋白中 6 个连续组氨酸残基 (His6) 的抗原表位。它与天然的和变性的组氨酸标记的融合蛋白反应，与表位序列位置无关。
应用
Anti-His 6 用于检测 His 6 标记的蛋白：
- 免疫印迹（例如：斑点印迹和免疫印迹）
- 免疫沉淀
- 免疫分析 (ELISA)
- 免疫细胞化学
- 免疫荧光
制备说明
稳定剂： BSA
工作浓度： 结合物的工作浓度取决于应用和底物。
以下浓度应作为指导：
工作浓度： 结合物的工作浓度取决于应用和底物。
以下浓度应作为指导：
- 免疫分析：检测为 0.1μg/ml；涂覆为 1-5μg/ml
- 免疫沉淀：0.5～5μg/ml
- Western 印迹法：0.2～0.5μg/ml
重悬
加入 1 mL 双蒸水至终浓度 100 μg/ml。
其他说明
仅用于生命科学研究。不可用于诊断。
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警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Skin Sens. 1
WGK
WGK 2
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
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