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形式
solution
分子量
239.5
包装
pkg of 2 × 25 mL (1 g)
制造商/商品名称
Roche
浓度
0.1-1.0 mg/mL
技术
electrophoresis: suitable
mp
175-185 °C
溶解性
water: soluble 0.1 M
储存温度
2-8°C
一般描述
Set consists of Pefabloc® SC and the corresponding amount of PLUS additive to avoid covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH. Choose Pefabloc® SC for all applications where the general inhibition of serine proteases is desired. With its high stability and irreversible inhibition mechanism, Pefabloc® SC protects proteins throughout procedures such as:
Use Pefabloc® SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE). With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc® SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis.
- Extraction processes (from animal tissues or cells, plants, bacteria, yeast, and fungi)
- Subsequent purification steps
- Varied sample storage conditions
- Downstream protein detection (e.g., western blotting, reporter gene analysis, etc.)
- Biochemical studies where proteins are required.
Use Pefabloc® SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE). With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc® SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis.
The Pefabloc® SC PLUS set combines the serine protease inhibitor Pefabloc® SC with a uniquely formulated Pefabloc® SC protector (PSC-Protector), adding additional convenience and reliability to the safe, stable protease inhibition provided by Pefabloc® SC .
Recent findings indicate that sulfonyl-type serine protease inhibitors such as Pefabloc® SC and PMSF can bind covalently to proteins. This can occur when the inhibitors are used in high concentrations, or during extended incubation times under alkaline conditions. This interaction adversely affects the tyrosine and lysine residues of a protein, as well as the free amino terminus. The PLUS additive (PSC-Protector) included with this set prevents covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH.
Recent findings indicate that sulfonyl-type serine protease inhibitors such as Pefabloc® SC and PMSF can bind covalently to proteins. This can occur when the inhibitors are used in high concentrations, or during extended incubation times under alkaline conditions. This interaction adversely affects the tyrosine and lysine residues of a protein, as well as the free amino terminus. The PLUS additive (PSC-Protector) included with this set prevents covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH.
特异性
Specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity of Pefabloc® SC is comparable to PMSF or DFP; however, Pefabloc® SC is non-toxic. Specifically inhibits serine proteases and prevents nonspecific covalent modification of proteins as tested by mass spectrometry.
特点和优势
- Take advantage of a simplified two-reagent system. Use the PSC-Protector solution to prevent covalent binding between proteins and Pefabloc® SC, even at high concentrations, extended incubation times, and at alkaline pH (see Figure 1 and Figure 2).
- Benefit from an easy-to-use inhibitor. Add water-soluble Pefabloc® SC directly to aqueous buffers.
- Avoid hazardous compounds. Obtain non-toxic protease inhibition without risk to you, or those around you.
- Ensure protection with improved stability. Achieve consistent protease inhibition even at pH levels above 7.0 and temperatures above +4°C.
- Maximize inhibition. Be certain that your levels of active inhibitor are high enough by using a product that is reliably soluble and stable.
包装
Set consists of Pefabloc® SC and the corresponding amount of PLUS additive to avoid covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH. Choose Pefabloc® SC for all applications where the general inhibition of serine proteases is desired. With its high stability and irreversible inhibition mechanism, Pefabloc® SC protects proteins throughout procedures such as:
- Extraction processes (from animal tissues or cells, plants, bacteria, yeast, and fungi)
- Subsequent purification steps
- Varied sample storage conditions
- Downstream protein detection (e.g., western blotting, reporter gene analysis, etc.)
- Biochemical studies where proteins are required.
Set containing two components
其他说明
仅用于生命科学研究。不可用于诊断。
法律信息
Pefabloc is a registered trademark of Pentapharm
仅试剂盒组分
产品编号
说明
- Pefabloc SC, 1 g
- PSC-Protector solution, 2 x 25 mL ready-to-use
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1B
WGK
WGK 1
法规信息
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