产品名称
抗HA亲和基质, from rat IgG1
biological source
rat
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F10, monoclonal
form
slurry
packaging
pkg of 1 mL (settled resin volume)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
capacity
2-8 nmol/mL binding capacity
storage temp.
2-8°C
Quality Level
Application
在以下实验中使用抗HA亲和基质：
- 来自哺乳动物，细菌和酵母细胞提取物的HA标记蛋白免疫沉淀
- 亲和柱纯化粗蛋白提取HA标记蛋白
在免疫沉淀或纯化之后，感兴趣的标记蛋白可以用于下列分析：
- 使用抗-HA抗体Western印迹
- 银染色（或相似的蛋白质染色）
- 免疫共沉淀。
Analysis Note
使用含有HA标记细菌碱性磷酸酶的全细胞细菌提取液能够获得9nmol纯化蛋白/ml亲和基质。
每一个批次的抗-HA亲和基质都经过HA标记蛋白纯化能力测试。这些蛋白是来自粗哺乳动物提取液感染细胞稳定表达蛋白。使用抗HA，高亲和抗体，western印迹法进行免疫沉淀。
Biochem/physiol Actions
抗-HA（3F10）识别9-氨基酸序列，YPYDVPDYA，这一序列来自人类流感血凝素（HA）蛋白。无论位置在哪儿（N末端，C末端或是中间），该抗原决定簇都可以被来自哺乳动物，细菌和酵母表达载体的融合蛋白识别。
Features and Benefits
- 获得高特异性和抗-HA高亲和还原交叉反应性。
- 使用非变性条件，洗脱目的HA标记蛋白。
- 纯化稀有HA标记表达蛋白。
- 对YPYDVPDYA有高特异性。
- 适合纯化含有HA抗原决定簇的蛋白，可以是N端，C端或中间融合。
- 适用于哺乳动物，细菌和酵母表达体系。
General description
抗-HA（3F10）识别9-氨基酸序列，YPYDVPDYA，这一序列来自人类流感血凝素（HA）蛋白。无论位置在哪儿（N末端、C末端或是中间），该抗原决定簇都可被来自哺乳动物、细菌和酵母表达载体的融合蛋白识别。
Immunogen
98-106氨基酸来自人类流感病毒血凝素蛋白
Other Notes
仅用于生命科学研究。不可用于诊断。
Physical form
PBS中1ml固定树脂抗-HA亲和基质，含有0.09%叠氮化钠（w/v);2ml重悬液等于1ml床柱。一个塑料柱包含顶端和底部的盖子。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
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实验方案
Anti-HA Affinity Matrix Protocol & Troubleshooting
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