产品名称
PCR 缓冲液不含 MgCl2,10x 浓缩, solution, pkg of 3 × 1 mL
form
solution
packaging
pkg of 3 × 1 mL
manufacturer/tradename
Roche
storage temp.
−20°C
Quality Level
Application
不含 MgCl2 的 PCR 缓冲液已用于通过聚合酶链反应(PCR)扩增特异性 DNA 片段。
General description
PCR缓冲液不含MgCl2,可与 MgCl2 储备液一起用于 PCR 反应,单独调整 Mg2+浓度。大多数应用中,1.5 mM MgCl2即可实现满意的结果,dNTP 浓度均为200μM。 不过,在许多情况下,需要通过滴定获得最佳Mg2+ 浓度,以提高 PCR 反应的特异性和产量。缓冲液为10 倍浓缩液,应与 MgCl2 2储备液一起使用。
Other Notes
供一般实验室使用。
Physical form
溶液,100 mM Tris-HCl,500 mM KCl (pH 8.3,20°C)
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Rao L Divi et al.
Toxicological sciences : an official journal of the Society of Toxicology, 118(1), 191-201 (2010-08-13)
Mitochondrial compromise has been documented in infants born to women infected with the human immunodeficiency virus (HIV-1) who received nucleoside reverse transcriptase inhibitor (NRTI) therapy during pregnancy. To model these human exposures, we examined mitochondrial integrity at birth and 1
Omid Yeganeh et al.
Iranian Red Crescent medical journal, 15(4), 340-344 (2013-10-02)
Chlamydia trachomatis and Mycoplasma genitalium infections are the most prevalent sexually transmitted bacterial infections in the world that cause urogenital infections in both men and women. It appears that infertility is a complication of these infections. This study was designed
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