一般描述
PCR缓冲液不含MgCl2,可与 MgCl2 储备液一起用于 PCR 反应,单独调整 Mg2+浓度。大多数应用中,1.5 mM MgCl2即可实现满意的结果,dNTP 浓度均为200μM。 不过,在许多情况下,需要通过滴定获得最佳Mg2+ 浓度,以提高 PCR 反应的特异性和产量。缓冲液为10 倍浓缩液,应与 MgCl2 2储备液一起使用。
应用
不含 MgCl2 的 PCR 缓冲液已用于通过聚合酶链反应(PCR)扩增特异性 DNA 片段。
外形
溶液,100 mM Tris-HCl,500 mM KCl (pH 8.3,20°C)
其他说明
供一般实验室使用。
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
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