生物来源
mouse
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
12CA5, monoclonal
检测方案
≥95% (HPLC and SDS-PAGE)
形式
solution
包装
pkg of 50 μg (500 μl)
制造商/商品名称
Roche
浓度
0.1 mg/mL
同位素/亚型
IgG2bκ
表位序列
YPYDVPDYA
储存温度
−20°C
一般描述
抗HA-过氧化物酶是与过氧化物酶偶联的HA肽的单克隆抗体。抗HA-过氧化物酶可识别源自人流感病毒血凝素蛋白(氨基酸98-106)的HA九肽序列(YPYDVPDYA)。即使通过称为“表位标记”的技术将HA肽表位引入不相关的重组蛋白中,抗体也可识别其抗原决定簇。
特异性
抗HA-过氧化物酶可识别来自人流感血凝素(HA)蛋白的9-氨基酸序列YPYDVPDYA。无论该表位位置如何(N末端、C末端或内部),其也可在融合蛋白中被识别。
该抗体与HA表位反应,HA表位是衍生自流感血凝素蛋白的九肽序列(YPYDVPDYA)。
免疫原
来自人流感病毒血凝素蛋白的氨基酸98-106
应用
- 通过以下方法,使用抗HA过氧化酶对天然流感血凝素蛋白和重组HA标签蛋白进行免疫化学检测:
- 斑点杂交法
- 蛋白质印迹法
注释:对于灵敏度不重要的实验,请使用抗HA过氧化酶(12CA5)。在低10倍浓度的蛋白质印迹中,为获得更高灵敏度的检测,请使用抗-HA高亲和力。
质量
通过测试每批抗HA抗体相对于标准品的功能性和纯度,以确认每个新试剂制剂的质量。
制备说明
稳定剂:BSA,蔗糖,0.02% 百里酚
工作浓度:偶联物的工作浓度取决于应用和底物。
以下浓度应作为使用指南:
工作溶液:Western封闭试剂在1x PBS中的20倍稀释液。
工作浓度:偶联物的工作浓度取决于应用和底物。
以下浓度应作为使用指南:
- 蛋白质印迹法:0.1 μg/ml
工作溶液:Western封闭试剂在1x PBS中的20倍稀释液。
其他说明
仅用于生命科学研究。不可用于诊断。
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WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
含少量动物源组分生物产品
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