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Merck
CN

11467140001

Roche

DNA,MB级

from fish sperm

别名:

DNA

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About This Item

UNSPSC代码:
41106310

生物来源

fish (sperm)

质量水平

等级

Molecular Biology

形式

solution (ready-to-use)

分子量

40-1,000 kDa

包装

pkg of 500 mg

制造商/商品名称

Roche

浓度

100 μg/mL

颜色

light yellowopalescent

溶解性

water: miscible

储存温度

−20°C

一般描述

MB级DNA是以超声的变性单链DNA片段混合物形式提供的。
MB级DNA被用于防止非特异性杂交反应。

应用

该即用型溶液是直接添加到杂交混合液中的。它可防止在所有类型的膜(Southern和Northern blot应用)或原位DNA杂交中发生非特异性杂交。MB级的DNA已被用于染色质免疫沉淀(ChiP)分析。
注:加入NaCl、超声或打断以及变性都不是必需的。

序列

链长为 120 至 3,000 个核苷酸

外形

溶液,10 mg/ml ,10 mM Tris-HCl,10 mM NaCl,1 mM EDTA,pH 8.0中

制备说明

工作浓度:100μg/ml
该鱼精DNA应在预杂交溶液中以100 μg/ml(1:100稀释)的浓度进行使用。

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

No data available

闪点(°C)

No data available

法规信息

动植物源性产品

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Yu Sasano et al.
Bio-protocol, 7(10), e2306-e2306 (2017-05-20)
Chromosome engineering is an important technology with applications in basic biology and biotechnology. Chromosome splitting technology called PCS (PCR-mediated Chromosome Splitting) has already been developed as a fundamental chromosome engineering technology in the budding yeast. However, the splitting efficiency of
Ryudo Ohbayashi et al.
Bio-protocol, 8(15), e2958-e2958 (2018-08-05)
Cyanobacteria are prokaryotic organisms that carry out oxygenic photosynthesis. The fresh water cyanobacterium Synechococcus elongatus PCC 7942 is a model organism for the study of photosynthesis and gene regulation, and for biotechnological applications. Besides several freshwater cyanobacteria, S. elongatus 7942
Jasmin van den Heuvel et al.
eLife, 10 (2021-07-29)
In humans and other holozoan organisms, the ribosomal protein eS30 is synthesized as a fusion protein with the ubiquitin-like protein FUBI. However, FUBI is not part of the mature 40S ribosomal subunit and cleaved off by an as-of-yet unidentified protease.
Dilip Kumar et al.
Journal of immunology (Baltimore, Md. : 1950), 182(2), 1011-1020 (2009-01-07)
The MAPKs ERK, JNK, and p38 control diverse aspects of the immune response, including regulation of cytotoxin biology in NK cells and CTL. The chemokine CCL5 is coreleased with the cytotoxins, perforin, the granzymes, and granulysin, during the lethal hit

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