Interleukin-1β, human (hIL-1β)

Recombinant Interleukin-1β, human (hIL-1β) is produced in E. coli and purified by standard chromatographic techniques.

Contents
100,000 U/ml (2 μg/ml) in PBS (phosphate buffered saline) and 1 mg/ml BSA (bovine serum albumin), [purity of BSA: >98%, endotoxin (LAL): <1 EU/mg BSA], filtered through 0.2 μm pore size membrane.

Interleukin-1 (IL-1), secreted by activated monocytes or macrophages and other cell types, is a pleiotropic factor for a variety of sensitive cells.

Recombinant IL-1β, human is effective on mouse and human cells. Interleukin-1 (IL-1) is produced by a number of cell types, including activated macrophages, B-cells, fibroblasts, and keratinocytes. It mediates a wide range of biological activities, such as stimulation of thymocyte proliferation via induction of interleukin-2 (IL-2) release, stimulation of B-lymphocyte maturation and proliferation, fibroblast growth factor activity, and induction of acute-phase protein synthesis by hepatocytes. IL-1 has also been reported to stimulate prostaglandin and collagenase release from synovial cells, and to be identical to endogenous pyrogen and catabolin.
Two types of human interleukin-1 (hIL-1α and hIL-1β) have been described.
Both types of hIL-1 stimulate proliferation and differentiation of T- and B-lymphocytes. Recently, it has been shown that two different high-affinity IL-1 receptor molecules exist on different cell types. In both, human and mouse, T-cells and fibroblasts express an 80 kDa receptor molecule, whereas an 60 kDa receptor molecule was found on B-cells and neutrophils. The action of hIL-1a and hIL-1β is mediated by both receptor molecules. IL-1 appears to have a wide range of stimulatory effects on the maturation, differentiation, and growth of many cell types involved in inflammation and development. Cells whose growth is directly or indirectly stimulated by IL-1 are fibroblasts, synovial cells, endothelial cells, epithelial cells, bone marrow cells, T-lymphocytes, and B-lymphocytes.

Working concentration: 5-500 U/ml
hIL-1β exerts its biological activity in the concentration range of 5–500 U/mI (0.1–1 ng/ml).
Working solution: Dilute the concentrated IL-1β solution (100,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 –10% serum.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

For life science research only. Not for use in diagnostic procedures.

One polypeptide chain (154 amino acids), identical to natural human IL-1β (153 amino acids), but with an extra methionine at the amino-terminus.

Chain Length 154 AA

EC₅₀ definition: The amount of hIL-1β that is required to support half-maximal stimulation of DNA synthesis (BrdU incorporation) with mouse C3H/HeJ thymocytes in the presence of saturating amounts of hIL-2 (50 U/ml, 25 ng/ml) (1 unit equals ≤0.02 ng).