一般描述
BM紫是碱性磷酸酶（AP）的显色底物，其设计用于沉淀酶免疫测定。它可在膜或固体支持物上的AP结合位点处形成永久的深紫色条带或斑点。该试剂不需要进行混合或溶解，其是一种稳定的底物溶液可直接从瓶中使用。与使用BCIP/NBT双瓶系统相比，它产生更少的背景染色，且不会降低灵敏度。反应产物呈深紫色，不溶于水。形成的深紫色可通过视觉观察到。
使用前外观： 淡黄色;
颜色显影后：深紫色
使用前外观： 淡黄色;
颜色显影后：深紫色
应用
- 比色AP底物，可用于：免疫组化
- Western印迹
- Southern印迹
- Northern印迹
- 菌落和噬菌斑杂交
- 原位 杂交
组分
即用型溶液。
制备说明
工作浓度：使用200 mU POD和酪蛋白作为封闭试剂可获得了最佳的结果。
工作溶液：在水中的溶解度：不溶。
工作溶液：在水中的溶解度：不溶。
其他说明
仅用于生命科学研究。不可用于诊断。
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
Valentina Garcia-Lee et al.
The International journal of developmental biology, 65(4-5-6), 395-401 (2020-09-16)
The cell differentiation of the musculoskeletal system is highly coordinated during limb development. In the distal-most region of the limb, WNT and FGF released from the apical ectodermal ridge maintain mesenchymal cells in the undifferentiated stage. Once the cells stop
Christoph Kessle
Nonradioactive Analysis of Biomolecules null
Jonas Malkmus et al.
Nature communications, 12(1), 5557-5557 (2021-09-23)
Precise cis-regulatory control of gene expression is essential for normal embryogenesis and tissue development. The BMP antagonist Gremlin1 (Grem1) is a key node in the signalling system that coordinately controls limb bud development. Here, we use mouse reverse genetics to
Deepika Sharma et al.
eLife, 10 (2021-10-15)
Osteoblast differentiation is sequentially characterized by high rates of proliferation followed by increased protein and matrix synthesis, processes that require substantial amino acid acquisition and production. How osteoblasts obtain or maintain intracellular amino acid production is poorly understood. Here, we
Ning Pan et al.
PloS one, 7(1), e30358-e30358 (2012-01-27)
Atonal homolog1 (Atoh1) is a bHLH transcription factor essential for inner ear hair cell differentiation. Targeted expression of Atoh1 at various stages in development can result in hair cell differentiation in the ear. However, the level and duration of Atoh1
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