推荐产品
生物来源
sheep
质量水平
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
表单
lyophilized (stabilized)
包装
pkg of 150 U
制造商/商品名称
Roche
储存温度
2-8°C
一般描述
辣根过氧化物酶标记的抗荧光素多克隆抗体的Fab片段。该试剂是辣根过氧化物酶（POD）标记的绵羊抗荧光素抗体Fab片段。用抗荧光素免疫后，采用离子交换色谱法纯化绵羊IgG（免疫球蛋白G），然后采用免疫吸附法分离特异性IgG。采用凝胶过滤法对通过木瓜蛋白酶消化得到的Fab片段进行纯化，将其与POD偶联，然后稳定在含0.4％牛免疫球蛋白（w/v）和0.2％ Germall II（w/v）、pH 7.2的60 mM Tris-Hepes缓冲液中。
特异性
该多克隆抗体可与游离和结合的荧光素反应。
应用
抗荧光素-POD Fab片段可用于以下方法检测荧光素标记化合物：
- 斑点杂交法
- ELISA（酶联免疫吸附测定）
- 免疫组织化学
- 原位 杂交
- Southern印迹
- 蛋白质印迹
制备说明
工作浓度：结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导：
工作溶液: 100 mM Tris-HCl，150 mM NaCl，pH 7.5必要时可使用1%封闭试剂 (w/v)、干奶粉、1-5%热灭活胎牛血清（v/v）或绵羊正常血清，以减少非特异性结合。
- 斑点印迹：150 mU/ml
- ELISA: 50至150 mU/ml
- 免疫组织化学：250至500 mU/ml
- 原位杂交：1.5至7.5 mU/ml
- Southern blot：150 mU/ml
- Western blot：500至1000mU/ml
工作溶液: 100 mM Tris-HCl，150 mM NaCl，pH 7.5必要时可使用1%封闭试剂 (w/v)、干奶粉、1-5%热灭活胎牛血清（v/v）或绵羊正常血清，以减少非特异性结合。
重悬
加入1ml双蒸馏水，最终浓度为150 U/ml。
其他说明
仅用于生命科学研究。不可用于诊断。
未找到合适的产品？
试试我们的产品选型工具.
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
含少量动物源组分生物产品
常规特殊物品
Alemji A Taku et al.
Development (Cambridge, England), 143(1), 123-132 (2016-01-07)
Olfactory sensory axons target well-defined intermediate targets in the zebrafish olfactory bulb called protoglomeruli well before they form odorant receptor-specific glomeruli. A subset of olfactory sensory neurons are labeled by expression of the or111-7:IRES:GAL4 transgene whose axons terminate in the
Iku Tsutsui-Kimura et al.
Nature communications, 8, 14304-14304 (2017-02-02)
Impaired motivation is present in a variety of neurological disorders, suggesting that decreased motivation is caused by broad dysfunction of the nervous system across a variety of circuits. Based on evidence that impaired motivation is a major symptom in the
Sandeep Gupta et al.
Developmental biology, 366(2), 125-141 (2012-04-28)
The mammalian hippocampus, a center of neurogenesis in the adult brain, is involved in critical functions such as learning and memory processing. Although there is an overall functional conservation between birds and mammals in the hippocampal region of the brain
Lisa A Thomas et al.
The Journal of comparative neurology, 510(1), 47-67 (2008-07-11)
Cell-cell interactions through adhesion molecules play key roles in the development of the nervous system. Synaptic cell adhesion molecules (SynCAMs) comprise a group of four immunoglobulin (Ig) superfamily members that mediate adhesion and are prominently expressed in the brain. Although
Johannes Kohl et al.
Nature, 556(7701), 326-331 (2018-04-13)
Parenting is essential for the survival and wellbeing of mammalian offspring. However, we lack a circuit-level understanding of how distinct components of this behaviour are coordinated. Here we investigate how galanin-expressing neurons in the medial preoptic area (MPOAGal) of the
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门