推荐产品
生物来源
sheep
质量水平
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
形式
lyophilized (stabilized)
包装
pkg of 150 U
制造商/商品名称
Roche
储存温度
2-8°C
一般描述
辣根过氧化物酶标记的抗荧光素多克隆抗体的Fab片段。该试剂是辣根过氧化物酶（POD）标记的绵羊抗荧光素抗体Fab片段。用抗荧光素免疫后，采用离子交换色谱法纯化绵羊IgG（免疫球蛋白G），然后采用免疫吸附法分离特异性IgG。采用凝胶过滤法对通过木瓜蛋白酶消化得到的Fab片段进行纯化，将其与POD偶联，然后稳定在含0.4％牛免疫球蛋白（w/v）和0.2％ Germall II（w/v）、pH 7.2的60 mM Tris-Hepes缓冲液中。
特异性
该多克隆抗体可与游离和结合的荧光素反应。
应用
抗荧光素-POD Fab片段可用于以下方法检测荧光素标记化合物：
- 斑点杂交法
- ELISA（酶联免疫吸附测定）
- 免疫组织化学
- 原位 杂交
- Southern印迹
- 蛋白质印迹
制备说明
工作浓度：结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导：
工作溶液: 100 mM Tris-HCl，150 mM NaCl，pH 7.5必要时可使用1%封闭试剂 (w/v)、干奶粉、1-5%热灭活胎牛血清（v/v）或绵羊正常血清，以减少非特异性结合。
- 斑点印迹：150 mU/ml
- ELISA: 50至150 mU/ml
- 免疫组织化学：250至500 mU/ml
- 原位杂交：1.5至7.5 mU/ml
- Southern blot：150 mU/ml
- Western blot：500至1000mU/ml
工作溶液: 100 mM Tris-HCl，150 mM NaCl，pH 7.5必要时可使用1%封闭试剂 (w/v)、干奶粉、1-5%热灭活胎牛血清（v/v）或绵羊正常血清，以减少非特异性结合。
重悬
加入1ml双蒸馏水，最终浓度为150 U/ml。
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
含少量动物源组分生物产品
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