产品名称
抗荧光素-POD Fab片段, from sheep
biological source
sheep
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized (stabilized)
packaging
pkg of 150 U
manufacturer/tradename
Roche
storage temp.
2-8°C
Quality Level
Application
抗荧光素-POD Fab片段可用于以下方法检测荧光素标记化合物：
- 斑点杂交法
- ELISA（酶联免疫吸附测定）
- 免疫组织化学
- 原位 杂交
- Southern印迹
- 蛋白质印迹
Biochem/physiol Actions
该多克隆抗体可与游离和结合的荧光素反应。
General description
辣根过氧化物酶标记的抗荧光素多克隆抗体的Fab片段。该试剂是辣根过氧化物酶（POD）标记的绵羊抗荧光素抗体Fab片段。用抗荧光素免疫后，采用离子交换色谱法纯化绵羊IgG（免疫球蛋白G），然后采用免疫吸附法分离特异性IgG。采用凝胶过滤法对通过木瓜蛋白酶消化得到的Fab片段进行纯化，将其与POD偶联，然后稳定在含0.4％牛免疫球蛋白（w/v）和0.2％ Germall II（w/v）、pH 7.2的60 mM Tris-Hepes缓冲液中。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
工作浓度：结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导：
工作溶液: 100 mM Tris-HCl，150 mM NaCl，pH 7.5必要时可使用1%封闭试剂 (w/v)、干奶粉、1-5%热灭活胎牛血清（v/v）或绵羊正常血清，以减少非特异性结合。
- 斑点印迹：150 mU/ml
- ELISA: 50至150 mU/ml
- 免疫组织化学：250至500 mU/ml
- 原位杂交：1.5至7.5 mU/ml
- Southern blot：150 mU/ml
- Western blot：500至1000mU/ml
工作溶液: 100 mM Tris-HCl，150 mM NaCl，pH 7.5必要时可使用1%封闭试剂 (w/v)、干奶粉、1-5%热灭活胎牛血清（v/v）或绵羊正常血清，以减少非特异性结合。
加入1ml双蒸馏水，最终浓度为150 U/ml。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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